动物营养学报Issue(2)：627-634,8.DOI:10.3969/j.issn.1006-267x.2016.02.039
谷氨酰胺及其前体物对松浦镜鲤组织抗氧化能力及血清生化指标的影响
Effects of Glutamine and Its Precursors on Tissue Antioxidant Capacity and Serum Biochemical Indices of Songpu Mirror Carp
摘要
Abstract
This experiment was conducted to study the effects of glutamine ( Gln) and its precursors on tissue antioxidant capacity and serum biochemical indices of Songpu mirror carp.A total of 1 050 Songpu mirror carp with an average body weight of (40.27±3.96) g were randomly selected and divided into 7 groups with 5 rep-licates per group, and 30 fish in each replicate.The fish in those groups were fed 7 isonitrogenous and isocalor-ic diets with 1.5%glucose ( control) , glutamine ( Gln) , glutamate ( Glu) , alpha-ketoglutarate ( AKG) , orni-thine-alpha-ketoglutarate ( OKG ) , arginine-alpha-ketoglutarate ( AAKG ) and sodium alpha-ketoglutarate di-basic ( 2Na-AKG) , respectively.The feeding trial lasted for 8 weeks.The results showed as follows: the su-peroxide dismutase ( SOD ) activity of the Gln and Glu groups was significantly higher than that of control group ( P<0.05) , the content of glutathione ( GSH) of the Gln group was significantly higher than that of con-trol group ( P<0.05) , the content of malondialdehyde ( MDA) of the Glu, AKG, OKG and 2Na-AKG groups was significantly lower than that of control group ( P<0.05) in intestine;the SOD activity of the Gln and Glu groups was significantly higher than that of other groups ( P<0.05) , the content of GSH had no significant difference among the groups ( P>0.05) , the content of MDA of the Gln, Glu, AKG, 2Na-AKG and OKG groups was significantly lower than that of control group ( P<0.05) in liver;the SOD activity of the OKG and 2Na-AKG groups was significantly higher than that of control group (P<0.05), the content of GSH of the 2Na-AKG group was significantly lower than that of control group ( P<0.05) , the content of MDA had no sig-nificant difference among the groups ( P>0.05) in serum.Compared with the control group, the serum TP content of the Gln, Glu and AKG groups was significantly increased ( P<0.05) , and that of 2Na-AKG group was significantly decreased ( P<0.05); the serum alanine transarninase ( ALT) activity of the AAKG group was significantly increased ( P<0.05) , the serum aspartate aminotransferase ( AST) activity of the OKG and 2Na-AKG groups was significantly increased ( P<0.05) and that of the AKG group was significantly decreased ( P<0.05); the serum total cholesterol ( TCHO) content of the AAKG group was significantly decreased ( P<0.05); the serum glucose content of the AKG group was significantly decreased (P<0.05).In conclusion, Gln and its precursors have some effect on tissue antioxidant capacity, and AKG, OKG, 2Na-AKG are better than others on removal of lipid peroxidation products; Gln, Glu and AKG can improve the protein utilization and immunity, moreover, AKG can reduce the blood glucose content.关键词
松浦镜鲤/谷氨酰胺/前体物/抗氧化能力/血清生化指标Key words
Songpu mirror carp/glutamine/precursor/antioxidant capacity/serum biochemical indices分类
农业科技引用本文复制引用
宋芳杰,王连生,徐奇友..谷氨酰胺及其前体物对松浦镜鲤组织抗氧化能力及血清生化指标的影响[J].动物营养学报,2016,(2):627-634,8.基金项目
中国水产科学研究院基本科研业务费资助（2014A08XK03）；国家大宗淡水鱼产业技术体系 （）
