福建农林大学学报（自然科学版）Issue(1)：8-13,6.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.002
南亚实蝇成虫肠道细菌的分离与鉴定
Isolation and identification of bacteria in the intestinal tract of adult Bactrocera tau (Walker) (Diptera:Tephritidae)
摘要
Abstract
21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16S rDNA ( V6-V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum, which included Enterobacter (7 strains), Raoultella (3 strains), Serratia ( 2 strains) , Providencia ( 2 strains) , Acinetobacter ( 1 strain) , Citrobacter ( 1 strain) , Cedecea ( 1 strain) and uncultured bacteria (4 strains). Similarly, 20 bacteria strains were isolated and identified from male B.tau and they also belonged to Gamma-proteobacteria phylum, which consisted of bacteria (7 strains), Klebsiella (4 strains), Enterobacter (3 strains), Providencia (2 strains), Serratia (1 strain ), Morganella (1 strain), Pseudomonas (1 strain ) and gamma Proteobacterium (1 strain). Bacteria genus which both females and males had in common were Enterobacter, Providencia and Serratia, which all belonged to Enterobacte-riaceae. To summarize, Enterobacteriaceae was the dominant bacterium in intestinal tract of B.tau.关键词
南亚实蝇/肠道/细菌/肠杆菌科Key words
Bactrocera tau/intestinal tract/bacterium/Enterobacteriaceae分类
生物科学引用本文复制引用
骆米娟,张贺贺,陈家骅,杜迎刚,何龙艳,季清娥..南亚实蝇成虫肠道细菌的分离与鉴定[J].福建农林大学学报（自然科学版）,2016,(1):8-13,6.基金项目
国家自然科学基金项目(11175046) （11175046）
