福建农林大学学报（自然科学版）Issue(1)：70-76,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.012
小花棘豆中毒对大鼠下丘脑-垂体-卵巢轴α-甘露糖苷酶的影响
Toxicity of Oxytropis glabra DC on α-mannosidase of hypothalamus-pituitary-ovarian axis in rats
摘要
Abstract
In order to investigate the toxicity mechanism of Oxytropis glabra DC, 144 3-month female rats were randomized into 4 groups (control group and experimental groupⅠ,Ⅱ,Ⅲ) and fed with mixture of O. glabra DC (fully ground above-ground part) and swainsonine ( 0, 30, 60 and 90 mg•kg-1 ) accordingly, until typical toxicity symptoms occurred. Then rats were selected ran-domly from each group every 7 d until the 63rd day. Hypothalamus, pituitary and ovary were collected, and contents of AMAⅠ,Ⅱand its mRNA expression in different encephalic regions were measured. Golgi AMA I and lysosomal AMA Ⅱ were detected in HPOA in four experimental groups. And the concentration of AMA1 and AMA2 mRNA decreased as doses of O. glabra DC in-creased. The difference was not significant in group Ⅰ and the control group in HPOA (P>0.05), but AMA1 and AMA2 mRNA were significantly lower in experimental groupⅡandⅢthan the control group( P<0.05) . And the inhabitation effect intensified as the study proceeded. To summarize, O. glabra DC reduces the concentration and transcriptional expression of AMA in female rat HPOA.关键词
小花棘豆/苦马豆素/α-甘露糖苷酶/下丘脑-垂体-卵巢轴Key words
Oxytropis glabra DC/swainsonine/α-mannosidase/hypothalamus-pituitary-ovarian axis分类
农业科技引用本文复制引用
王帅,贾琦珍,陈根元,程勇,张玲,马春晖..小花棘豆中毒对大鼠下丘脑-垂体-卵巢轴α-甘露糖苷酶的影响[J].福建农林大学学报（自然科学版）,2016,(1):70-76,7.基金项目
国家自然科学基金(31460678)；兵团塔里木畜牧科技重点实验室开放课题(HS201207) （31460678）
