分析化学Issue(3)：391-395,5.DOI:10.11895/j.issn.0253-3820.150773
基于直立碳纳米管大面积金粒子的DNA生物传感器用于早幼粒白血病/维甲酸受体α融合基因检测
DNA Biosensor Based on Large Area of Gold Particles on Aligned Carbon Nanotubes for Detection of Promyelocytic Leukaemia/Retinoic Acid Receptor αFusion Gene
摘要
Abstract
A novel electrochemical DNA biosensor was fabricated based on the large area of gold particles on the aligned carbon nanotubes for the detection of promyelocytic leukaemia/retinoic acid receptor α ( PML/RARα) fusion gene in acute promyelocytic leukemia. Firstly, the thiol-modified single-stranded DNA ( ssDNA, probe 1) was immobilized on the gold particles sputtered on the aligned carbon nanotubes electrode by self-assembly technique. Then, amino-modified ssDNA and carboxyl-modified CdTe quantum dots ( QDs) were combined to get CdTe-modified DNA probe through an amidization reaction. After hybridization with target DNA, a sandwich-type assay structure was formed. Finally, the CdTe quantum dots captured on the electrode surface were detected by differential pulse anodic stripping voltammetry. The change of peak current of Cd2+ on the electrode was found to be linear with the logarithm of target DNA concentration in the range of 1. 0×10-12 mol/L to 1. 0×10-8 mol/L. The Linear equation was ipa(μA)=1. 626+0. 132lgC (mol/L) (the correlation coefficient R was 0. 996) and the detection limit was 4. 0×10-13 mol/L (3σ). The fabricated DNA biosensor exhibited excellent reproducibility and stability.关键词
直立碳纳米管/金粒子/急性早幼粒细胞白血病/DNA生物传感器/差分脉冲阳极溶出伏安Key words
Aligned carbon nanotubes/Gold particles/Acute promyelocytic leukemia/DNA biosensor/Differential pulse anodic stripping voltammetry引用本文复制引用
杨丽珠,朱婧,章仁毅,马丹琦,何品刚,方禹之..基于直立碳纳米管大面积金粒子的DNA生物传感器用于早幼粒白血病/维甲酸受体α融合基因检测[J].分析化学,2016,(3):391-395,5.基金项目
本文系国家自然科学基金项目(No.31300819)和浙江省自然科学基金项目(No. LQ12B05004)资助@@@@This work was supported by the National Natural Science Foundation of China (No.31300819) and the Natural Science Foundation of Zhe-jiang Province, China (No. LQ12B05004) （No.31300819）
