分析化学Issue(3)：409-415,7.DOI:10.11895/j.issn.0253-3820.150769
全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素
An Automated On-line Solid Phase Extraction-Liquid Chromatography-Tandem Mass Spectrometric Method for Six Endogenous Phytohormones Analysis in Rice
摘要
Abstract
An automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry ( on-line SPE-LC-MS/MS ) method for the simultaneous quantification of six endogenous phytohormones including abscisic acid ( ABA ) , indole-3-acetic acid ( IAA ) , salicylic acid ( SA ) , jasmonic acid ( JA ) , indole-3-propionic acid ( IPA) and indole-3-butyric acid ( IBA) in rice tissues was described. Plant samples were extracted with methanol and on-line SPE procedure was performed with C18 SPE cartridges. Analytes were eluted to C18 analytical column with mobile phase and further analyzed by LC-MS/MS. The performance of the method was fully validated. Linearity showed good correlation (R2≥0. 99). Limits of detections (S/N=3) were in the range of 0. 1-0. 8 μg/kg. Recoveries varied between 71. 2% and 126%. The method was rapid and sensitive to determine multiple phytohormones in rice young panicles and the results were cross-validated with the off-line SPE-LC-MS/MS phytohormone analytical method. Finally, the method was applied to monitor the changes of ABA, IAA, SA and JA in wounded rice leaves. The trends were in accord with plant physiological processes.关键词
植物激素/在线固相萃取-液相色谱-串联质谱/定量分析/水稻Key words
Phytohormones/On-line solid phase extraction-liquid chromatography-tandem mass spectrometry/Quantitative analysis/Rice引用本文复制引用
夏群,辛培勇,褚金芳..全自动在线固相萃取-液相色谱-串联质谱法同时检测水稻中6种内源性植物激素[J].分析化学,2016,(3):409-415,7.基金项目
本文系国家自然科学基金(No.91417314)、中科院仪器设备功能开发技术创新项目和中科院关键技术人才项目资助@@@@This work was supported by the National Natural Science Foundation of China (No.91417314), and the Technique Innovation of Instrumental Function of CAS Program and CAS Key Technology Talent Program （No.91417314）
