分析化学Issue(3)：444-450,7.DOI:10.11895/j.issn.0253-3820.150831
间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析
Determination of Red Freesia Flower Volatiles with Indirect Headspace Solid Phase Microextraction Coupled to Gas Chromatography and Mass Spectrometry
摘要
Abstract
A method was established for the analysis of red Freesia flower volatiles by indirect headspace solid phase microextraction ( HS-SPME) . The gas collecting tube was used to enrich flower volatiles, and adsorbing material was resolved in the extraction solution. Then, the adsorbing material was analyzed by HS-SPME. The experimental conditions for the quantification of major flower volatiles ( linalool, terpineol, ionone and dihydrogen-ionone) were optimized. Florisil was used as adsorbent, with water-methanol solution (9∶1, V/V) and 0. 6 mol/L hydrochloric acid as the extraction solution. The thermal desorption temperature was 70℃ and the extraction time was 25 min. The results showed that the concentration range of linear relationship for linalool and terpineol ( R2≥0. 981 ) was 1-100 μg/mL, with detection limits of 0. 05 and 0. 10 μg, respectively, and 0. 5-50 μg/mL for dihydro ionone and ionone ( R2≥0. 988 ) with a detection limit of 0. 02 μg.关键词
红花香雪兰/花香成分/间接顶空固相微萃取/气相色谱-质谱Key words
Red Freesia/Flower volatiles/Indirect headspace solid phase microextraction/Gas chromatography-mass spectrometry引用本文复制引用
刘宝峰,高丰展,房强,王丽..间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析[J].分析化学,2016,(3):444-450,7.基金项目
本文系国家自然科学基金(Nos.31170276,31300271)资助项目@@@@This work was supported by the National Natural Science Foundation of China (Nos.31170276,31300271) （Nos.31170276,31300271）
