华北农学报Issue(z1)：1-7,7.DOI:10.7668/hbnxb.2015.S1.001
大肠杆菌 otsAB 融合基因表达载体的构建和转化小麦的初步研究
Preliminary Studyo n Construction of the Expression Vector otsAB Fusio n Gene in E.coli andT ransformation of Wheat
摘要
Abstract
The yield and quality of wheat,a kind of important food crops,are often influenced under the condi-tion of biotic or abiotic stresses.Genetic engineering technology is an effective method for wheat quality improve-ment,however,the transformation rate of wheat is still relatively low by far.In this experiment,E.coli genomic DNA was as template to clone ot sA and otsB genes by the mean of PCR method.Then the target genes were connected with p2300-GFP,which would lead to the construction of p2300-otsAB vector.On the basis of establishing a perfect regeneration system,the common wheat was as the material.The p2300-otsAB expression vector was introduced into the cells of mature embryo and callus of wheat by Agrobacterium-mediated method.And then the new variety of salt-tolerant transgenic wheat was obtained.The genetic transformation results showed that when the concentration of Agrobacterium was OD600 =0.5,the time of infection of mature embryo or callus was 30 min,and the co-culture time was 3 d,the transformation efficiency of wheat was the highest.The experimental results can provide the reference to improve the stress resistance of plants through genetic engineering in the future.关键词
小麦/otsA/otsB/成熟胚/愈伤组织/农杆菌Key words
Wheat/otsA gene/otsB gene/Mature embryo/Callus/Agrobacterium分类
农业科技引用本文复制引用
陆玉建,石东里,张兰,田莎,张韩杰,刘南南..大肠杆菌 otsAB 融合基因表达载体的构建和转化小麦的初步研究[J].华北农学报,2015,(z1):1-7,7.基金项目
山东省自然科学基金项目（ZR2012CL14）；滨州学院博士基金项目 （）
