华北农学报Issue(z1)：8-18,11.DOI:10.7668/hbnxb.2015.S1.002
高压静电场对羽衣甘蓝自交不亲和系种子基因组 DNA 甲基化的影响
Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala
摘要
Abstract
DNA methylation plays an important role in regulating plant growth,and it is necessary for plant nor-mal growth and development.In this experiment,methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incom-patible line 9# and the change of DNA methylation status in 9# after high-voltage electric field.The correlation was discussed between seed degradation phenomena of self-incompatible line and epigenetic one.The results were shown as follows:After high-voltage electric field treatment,both methylation and demethylation occurred;and demethyla-tion seemed to be predominant,the proportion of full-methylation increased slightly and semi-methylation declined clearly and brought about the result of total methylation declined;the physiological indexes of 9# in seed germina-tion,which showed high-voltage electric field,could promote seed germination and seeding growth and improve the quality of seeds.High-voltage electric field was an effective means to delay and restore growth vigor,and to maintain the quality of self-crossed progeny.关键词
羽衣甘蓝/自交不亲和性/DNA 甲基化/高压静电场Key words
Brassica oleracea var.acephala/Self-incompatibility/DNA methylation/High-voltage electric field分类
生物科学引用本文复制引用
张旸,胡中影,李娜,丁兵,王江,解莉楠..高压静电场对羽衣甘蓝自交不亲和系种子基因组 DNA 甲基化的影响[J].华北农学报,2015,(z1):8-18,11.基金项目
中央高校基本科研业务费专项资金项目（2013BAQ01）；哈尔滨市科技创新人才研究专项资金项目（青年科技创新人才） （）
