华北农学报Issue(z1)：38-45,8.DOI:10.7668/hbnxb.2015.S1.007
微卫星标记在濒危哲罗鱼增殖放流中的应用
Application of Microsatellite Markers in Endangered Hucho taimen Enhancement and Releasing
摘要
Abstract
In order to study the application of microsatellites markers in endangered Hucho taimen enhancement and releasing hucho releasing,eighteen microsatellites markers were used to analyze the genetic diversity and struc-ture of release-recapture population (JC),Tahe River population (TH) and released parents′population (HT,YZ) in the present study.The recaptured individuals were also identified.The results indicated that Na,Ne,I,He and H indexes exhibited the same tendency,HT >JC >TH >YZ.The observed heterozygosity (Ho) was highest in YZ population while lowest in TH population.All of these four populations possessed a medium level of genetic diversi-ty.AMOVA analysis showed that there was no significant difference within four groups while extremely significant difference between populations.The genetic similarity was highest between YZ and HT population with the index of 0.718 3,medium-level between JC population and other three populations with the index of 0.566 5 -0.689 5,and lowest between YZ and TH population with the index of 0.495 9.The results showed that we could understand the genetic background of the parents and assure the released descendant′s polymorphism by microsatellite markers.The released individuals were identified by microsatellite markers.In conclusion,this study provided the basis for the next stage of artificial releasing work.关键词
微卫星标记/哲罗鱼/增殖放流Key words
Microsatellite markers/Hucho taimen/Enhancement and releasing分类
农业科技引用本文复制引用
佟广香,匡友谊,张永泉,尹家胜..微卫星标记在濒危哲罗鱼增殖放流中的应用[J].华北农学报,2015,(z1):38-45,8.基金项目
国家科技支撑计划项目（2012BDA25B10）；黑龙江省科技计划项目（GZ11B201）；中央级公益性科研院所基本科研业务费专项（201109）；农业部淡水水产生物技术与遗传育种重点实验室开放课题 （）
