华北农学报Issue(z1):55-60,6.DOI:10.7668/hbnxb.2015.S1.010
不同倍性亚洲百合的花粉形态与活力变化规律分析
Analysis on Pollen Morphology and Variation of Viability of Lilium Asiatic Hybrids with Different Ploidy
摘要
Abstract
In order to investigate differences of pollen morphology and variation of viability of Lilium Asiatic hybrids in ploidy level,with 8 Asiatic lily cultivars as materials,pollen viability was measured by the methods of mixed dye staining and germination.Meanwhile,pollen morphology was observed with an electron microscope.The results showed that the highest pollen viability of three kinds of ploidy Asiatic lily appeared in the first day of the flowering period,which of the triploid varieties were at 10:30 AM,diploid and tetraploid cultivars were at 12:30 PM.Trends of pollen viability in 8 lily cultivars increased first and then decreased.There was a positive correlation between pollen size and ploidy.The polar axis ranged in 96.47 -137.91 μm and the equatorial axis value were 40.68 -56.55 μm.P ×E value of tetraploid pollen was the largest,followed by triploid and diploid.The pollen cen-tral mesh size range 3.93 to 8.57 μm,which increased with increasing ploidy of varieties.Morphological parameters cluster displayed higher pollen similarity in the same ploidy hybrids.The research showed that there was a big difference in the persistence of pollen viability between different ploidy of Asiatic lilies.According to the ploidy of varieties,breeders can carry out pollination in the period of peak viability of pollen.关键词
亚洲百合/品种/倍性/花粉活力/花粉形态Key words
Asiatic lily/Varieties/Ploidy/Pollen viability/Pollen morphology分类
农业科技引用本文复制引用
崔光芬,杜文文,段青,贾文杰,王祥宁..不同倍性亚洲百合的花粉形态与活力变化规律分析[J].华北农学报,2015,(z1):55-60,6.基金项目
云南省重点科技计划项目(2012BB011);云南省农科院基础预研项目 ()