华北农学报Issue(z1)：200-203,4.DOI:10.7668/hbnxb.2015.S1.036
芒果 CHS 基因的克隆及其表达分析
Cloning and Sequence Analysis of CHS Gene in Mango
摘要
Abstract
In order to study the function and molecular mechanism of CHS.A CHS gene was cloned from the mango fruit cDNA and DNA,by homology cloning method.The open reading frame was 1 194 bp,and encoding 397 amino acids,its isoelectric point was 6.03,the molecular weight was 43.29 kDa.The 1 315 bp fragment was ampli-fied from the genome,the result showed that the gene contained a intron by analysis.The expression of CHS gene was analysis in young leaves and mature leaves,it found that the gene was expressed in different period leaves,and higher in the red young leaves.It was inferred that the gene might be involved in the flavonoids synthesis of leaves.It was found that the gene encoding for the protein had close relationship with Paphiopedilum concolor,Bletilla striata, through phylogenetic analysis,considered as a group with Bletilla striata,Equisetum arvense and,Brassica oleracea.关键词
芒果/CHS/基因克隆/表达分析Key words
Mango/CHS/Gene cloning/Expression analysis分类
生物科学引用本文复制引用
赵志常,陈业渊,高爱平,黄建峰,党志国,罗睿雄..芒果 CHS 基因的克隆及其表达分析[J].华北农学报,2015,(z1):200-203,4.基金项目
国家自然科学基金项目（31471850）；农业部热带作物种质资源保护（15RZZY－07）；“948”项目（2011－G13）；非营利性科研机构改革启动经费项目 （）
