华北农学报Issue(z1)：225-230,6.DOI:10.7668/hbnxb.2015.S1.040
低温后不同光强对烤烟幼苗叶片活性氧代谢和叶绿素荧光参数的影响
Effects of Different Light Intensities on Reactive Oxygen Species Metabolism and Chlorophyll Fluorescence Parameters in Leaves of Flue-curing Tobacco Seedlings after Chilling Stress
摘要
Abstract
In this paper,the changes of reactive oxygen species and chlorophyll fluorescence parameters in leaves of flue-curing tobacco seedlings in different combinations of simulating low temperature (8 ±2)℃ and light intensity(weak light:200 μmol/(m 2・ s),strong light:1 000 μmol/(m 2・ s)) in the early spring of northern China after chilling stress were studied,the aim was to exploit response mechanism of regular low temperature and strong light on survival and growth of transplanting tobacco seedlings.The results showed that after low temperature for 1, 2 h,the rates of production of O 2 had not changed significantly in the weak light treatment,while,the rates of pro-・-duction of O 2 had increased significantly in the treatment of strong light.Meanwhile the produce rate of O・-2 and in-・-creasing contents of H2 O2 in strong light were obviously higher than in weak light.The activity of SOD and APX were increased significantly in weak light after low temperature for 1,2 h and in strong light after low temperature for 1 h,while,the activity of SOD and APX were decreased mostly in the strong light after low temperature for 2 h. The changes of reactivity oxygen species in tobacco leaves under different stress resulted in the numerous MDA ac-cumulation,the extent of membranous peroxide had been increased.Samely,the MDA contents in strong light treat-ment were more than in weak light treatment,and the MDA contents in low temperature for 2 h were more than for 1 h.The parameters of Fv/Fm and Fv/Fo were not changed significantly in weak light after low temperature for 1, 2 h,while,parameters of Fv /Fm and Fv/Fo were decreased mostly in the strong light after low temperature,and de-gree of decreasing in low temperature for 2 h were more than for 1 h.关键词
低温/光强/烤烟/活性氧/叶绿素荧光Key words
Low temperature/Light intensities/Flue-curing tobacco/Reactive oxygen species/Chlorophyll fluo-rescence分类
农业科技引用本文复制引用
郭瑞,曾榕,张金鑫,张秀丽,孙广玉..低温后不同光强对烤烟幼苗叶片活性氧代谢和叶绿素荧光参数的影响[J].华北农学报,2015,(z1):225-230,6.基金项目
哈尔滨市科技创新人才研究专项（2013RFXXJ063）；东北林业大学生命科学学院大学生创新训练计划项目 （）
