华北农学报Issue(z1)：342-347,6.DOI:10.7668/hbnxb.2015.S1.062
猪流行性腹泻病毒 TX 株的分离鉴定及毒力分析
Isolation,Identification and Virulence Analysis of Porcine Epidemic Diarrhea Virus TX Strain
摘要
Abstract
Porcine epidemic diahorrea virus (PEDV) TX strain was isolated from the feces of piglets suspected to be suffering from a severe diarrhea in Jiangsu Province.To investigate the occurrence and origin of diarrhea in piglet,porcine epidemic disease virus (PEDV),transmissible gastroenteritis coronavirus (TGEV) and porcine rotavirus (RV) et al were detected by RT-PCR.Typical coronavirus particles were observed by transmission electron micros-copy(TEM) in feces samples.The phylogenetic tree was established by comparing the S1 gene amplifying from TX strain with other PEDV strains.In order to identified the virus pathogenicity,age of 5,15,25 days piglet were chal-lenged with TX strain.The samples were only positive to PEDV detected by RT-PCR.The TX strain shared a highly homology of 94.8% -99.3% with epidemic strains.All the piglets died after were challenged with virus,indicating a high pathogenicity.关键词
猪流行性腹泻病毒/分离鉴定/进化分析/致病力Key words
Porcine epidemic diarrhea virus/Isolation and identification/Analysis of phylogenetic/Virulence分类
农业科技引用本文复制引用
于晓明,陈瑾,侯立婷,吴楠,侯继波,郑其升..猪流行性腹泻病毒 TX 株的分离鉴定及毒力分析[J].华北农学报,2015,(z1):342-347,6.基金项目
江苏省农业自主创新专项（CX（14）2089） （）
