华北农学报Issue(z1)：364-371,8.DOI:10.7668/hbnxb.2015.S1.066
小花棘豆中毒对大鼠下丘脑－垂体－睾丸轴α－甘露糖苷酶的影响
Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Hypothalamus-pituitary-testicular Axis in Rats
摘要
Abstract
The aim of this study was to investigate the effects of content and transcriptional expression of α-mannosidase(AMA) in hypothalamus-pituitary-testicular axis poisoning by Oxytropis glabra DC,and reveal the tox-icity mechanism of O.glabra DC in future.144 male rats were randomly and equally divided into 4 groups(the con-trol group and experimental groupⅠ,Ⅱ,Ⅲ),the dried plant of O.glabra DC was comminnuted,and different a-mounts of the grass powder(15%,30%and 45% the corresponding swainsonine content was 30,60 and 90 mg/kg) were mixed with the feeds in the three experimental groups.The rats consumed the forages freely until typical symp-toms were observed.4 rats were selected randomly from each group every 7 day respectively,the hypothalamus,pitu-itary and testis were collected and the contents of AMA and its mRNA expression in different encephalic regions were measured.It was found that AMA in golgi and lysosomal expressed in hypothalamus-pituitary-gonad of all ex-perimental groups, O.glabra DC inhibited the expression of hypothalamus-pituitary-testicular axis AMA1 and AMA2 mRNA,and the difference was obvious.The difference was not obvious in experimental groupⅠ and control group in hypothalamus-pituitary-testicular axis(P >0.05),the expression of AMA1 and AMA2 mRNA decreased in experi-mental group Ⅲ and AMA1 mRNA decreased in experimental groupⅡ was significant lower than control group in hypothalamus-pituitary-testicular axis ( P <0.05), and it was significant lower than control group.The results showed that O.glabra DC has remarkable effects on content and transcriptional expression of AMA in male rat hypo-thalamus-pituitary-testicular axis,in a time-effect as a dose-effect relationship.关键词
小花棘豆/苦马豆素/α-甘露糖苷酶/下丘脑-垂体-睾丸轴Key words
Oxytropis glabra DC/Swainsonine/α-mannosidase/Hypothalamus-pituitary-testicular axis分类
农业科技引用本文复制引用
王帅,贾琦珍,张玲,瞿胜,陈根元..小花棘豆中毒对大鼠下丘脑－垂体－睾丸轴α－甘露糖苷酶的影响[J].华北农学报,2015,(z1):364-371,8.基金项目
国家自然科学基金项目（31460678）；兵团塔里木畜牧科技重点实验室开放课题 （）
