华北农学报Issue(1)：15-21,7.DOI:10.7668/hbnxb.2016.01.003
陆地棉 EPSPS 基因全基因组分析
Genome-wide Analysis of the EPSPS Genes in Upland Cotton
摘要
Abstract
Currently,two EPSPS genes from Gossypium hirsutum have been submitted to NCBI.It is more con-vinent to identify EPSPS genes in genome level of upland cotton comprehensively following the publication of Gos-sypium hirsutum genomic sequence.Four EPSPS homologous genes were identified from the genome sequence data-base of allotetraploid cotton (Gossypium hirsutum L.acc.TM-1),and they were found to be distributed on subge-nomes of A07,A12,D07 and D12.Under the comperation of nucleotide sequences,amino acid sequences and gene structures,phylogenetic analysis of constructing phylogenetic tree and the study of transcription situation of these four genes,it was more clearly that the two genes located on A07 and D07 subgenomes belong to highly homologous co-linear gene,and the two genes located on A12 and D12 subgenomes as well.Sequence alignment results showed that the two EPSPS genes submitted to NCBI were the two genes located on A12 and D12 subgenomes respectively. The study results of this paper would provide an theoretical basis on cloning and functional research of EPSPS genes which located on A07 and D07 subgenomes.关键词
陆地棉/陆地棉基因组/EPSPS 基因Key words
Gossypium hirsutum/Genome sequence of upland cotton/EPSPS gene分类
农业科技引用本文复制引用
巩元勇,徐珍珍,郭书巧,束红梅,蒋璐,倪万潮..陆地棉 EPSPS 基因全基因组分析[J].华北农学报,2016,(1):15-21,7.基金项目
国家自然科学基金项目（31301682）；江苏省农业科技自主创新基金项目（CX（14）5009）；国家转基因重大专项 （）
