华北农学报Issue(1)：29-34,6.DOI:10.7668/hbnxb.2016.01.005
毒蛋白 A 链基因被共抑制的蓖麻种子中毒蛋白含量的测定
Determination of Toxic Protein Ricin a Chain Gene was Co-suppression of Seed Poisoning Protein Content
摘要
Abstract
The purpose of this experiment was to a chain gene toxic protein was co-suppressed in transgenic castor plants T0 ,T1 ,T2 seeds were determination of toxic protein content,obtain a low content of toxic protein ricin new transgenic material to lay the foundation.The results were as follows:we used phosphate extraction method,BCA kit method,UVwin5,ultravioletV5.1.0,BandScan protein quantitative analysis.,after obtained the two co-suppression stable genetic material,nameed T2-9-1,T2-15,A chain toxin protein content were 0.Contrast Tongliao 5 ricin B chain respectively 77.63%,83.38%,both materials could be used as low-toxic protein ricin material to promote.关键词
蓖麻/毒蛋白/A 链基因Key words
Castor/Ricin/A chain gene分类
农业科技引用本文复制引用
黄凤兰,雷雪,赵永,李国瑞,罗蕊,陈晓凤,李跃,孙华军..毒蛋白 A 链基因被共抑制的蓖麻种子中毒蛋白含量的测定[J].华北农学报,2016,(1):29-34,6.基金项目
国家自然科学基金项目（30760123；31160290；31460353）；国家农业部公益性行业项目（201003057）；内蒙古自治区高等学校“青年科技英才支持计划”（NJYT－14－A10）；内蒙古自治区科技创新引导奖励资金项目（201506）；内蒙古自治区“草原英才”计划项目（201511）；市校合作项目（ SXZD2012018）；内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目 （）
