华北农学报Issue(1)：51-56,6.DOI:10.7668/hbnxb.2016.01.009
水稻抗稻瘟病 Pigm（t）基因的分子标记辅助选择与利用
Marker-assisted Selection and Application of Blast Resistant Gene Pigm(t) in Rice
摘要
Abstract
This study was aimed to improve the resistance of Wuyunjing 29196.Blast resistant gene Pigm(t) showed a broad spectrum of strong resistance.Wuyunjing 29196 is an excellent conventional Japonica rice,which has good yielding but sensitive to rice blast.This study was aimed to improve the resistance of Wuyunjing 29196 to blast by MAS and anther culture.GM4,a variety carrying a resistant gene Pigm(t),was used as the donor parent. Two closely linked InDel markers,S95477 and S29742,were employed to select the Pigm(t) gene in each genera-tion.Finally,185 DH (Double haploid) lines were developed from the anthers of BC2 F1 plants.Among them,82 im-proved lines with homozygous Pigm(t) were selected.Multiple agronomic traits including grain yield and resistance to rice blast and the rice quality were characterized.Two fine lines,DH036 and DH158 screened out,finally which had increased resistance level and enhanced yield production.More importantly,they had similar plant architecture and other traits.Simultaneously had high resistance to rice blast and quality.The combination of anther culture and marker-assisted selection successfully improved the resistance to rice blast,in a short breeding period.The improved Wuyunjing 29196 with high blast resistance level provided important genetic resources in breeding.关键词
水稻/抗稻瘟病基因 Pigm(t)/分子标记辅助选择/回交育种/花药培养Key words
Rice/Pigm(t)/Marker assisted selection/Back cross breeding/Anther culture分类
生物科学引用本文复制引用
王飞,王立广,潘梅瑶,钮中一,周勇,梁国华..水稻抗稻瘟病 Pigm（t）基因的分子标记辅助选择与利用[J].华北农学报,2016,(1):51-56,6.基金项目
国家“973”项目（2013CBA01405）；国家自然科学基金项目（31100863；31271679）；江苏省普通高校专业学位研究生科研创新计划项目 （）
