华北农学报Issue(1)：77-82,6.DOI:10.7668/hbnxb.2016.01.013
葡萄逆境胁迫诱导启动子的克隆及表达分析
Cloning and Expression Analysis of Grape′s Stress Inducible Promoter
摘要
Abstract
To study the expression of grape stress tolerance gene (CAN70200.1),a 1 354 bp promoter frag-ment (named as PCAN ) upstream of the gene CAN70200.1 was isolated by using PCR technology.Promoter se-quence was analyzed by the database of PlantCARE and PLACE.The result showed that the PCAN sequence con-tained basic elements CAAT-box,TATA-box and some cis-acting elements that response to abiotic stresses,light and plant hormones.To verify the expression pattern of the promoter,the PCAN fragment was fused with GUS reporter gene located on pCAMBIA1391Z to construct a plant expression vector p1391Z-CAN,followed by transformation into to-bacco by Agrobacterium-meditated method.The expression activity of PCAN promoter reached highest at 120 min after drought stress treatment or at 30 -60 min under 4 ℃ cold treatment condition,indicated that the PCAN promoter could express under the condition of treatments with cold and drought.关键词
葡萄/PCAN启动子/胁迫诱导表达/GUS 检测Key words
Grape/PCAN promoter/Stress induction expression/GUS assay分类
生物科学引用本文复制引用
吕兆勇,赵春梅,薛仁镐..葡萄逆境胁迫诱导启动子的克隆及表达分析[J].华北农学报,2016,(1):77-82,6.基金项目
转基因生物新品种培育重大专项（2014ZX08010002－003－002）；山东省自然科学基金项目 （）
