江西农业大学学报Issue(1):54-59,6.DOI:10.13836/j.jjau.2016008
铜胁迫对小麦种子萌发及幼苗抗氧化系统的影响
Effects of Copper Stress on Germination and Antioxidant System in Wheat Seedings
摘要
Abstract
To illuminate the physiological mechanisms of copper tolerance of wheat,Hoagland nutrient cul-ture method was adopted to study the effect of stress of different Cu2+concentrations(0,10,20,50,70,100,150 mg/L CuSO4) on seed germination,bud growth,seedling chlorophyll content and antioxidant system.The re-sults showed that with the increase of copper concentration, wheat seeds germination rate rose first and then dropped.Root length,shoot length were decreased,and the effect of inhibitory on root was earlier than on bud. With Copper concentration increaseing, the chlorophyll content of wheat seedlings decreased gradually, and photosynthesis was inhibited.The CAT and SOD activity of treatments(50,70 mg/L of CuSO4 ) were increased significantly,and the copper-induced oxidative stress was relieved,the content of MDA was relativly decreased. The CuSO4,POD,CAT,SOD activity and MDA levels in the treatments(100,150mg/L) were significantly in-creased.The protective enzyme system was stimulated by excess copper, and the ROS in seedlings was in-creased,and membranous peroxidation was caused,and the growth of wheat was hindered.关键词
铜/小麦/种子萌发/抗氧化酶活性Key words
copper/wheat/germination/antioxidant enzyme activity分类
农业科技引用本文复制引用
薛盈文,王玉凤,赵长江,王智慧..铜胁迫对小麦种子萌发及幼苗抗氧化系统的影响[J].江西农业大学学报,2016,(1):54-59,6.基金项目
国家自然科学基金项目(31101429)和国家公益性行业科研专项(201303007)Project supported by the National Natural Science Foundation of China(31101429),National Special Fund for Agro-scientific Research in the Public Interest ()