江西农业大学学报Issue(1)：146-151,197,7.DOI:10.13836/j.jjau.2016021
小花棘豆中毒对和田羊脑组织α-甘露糖苷酶的影响
Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Brain in Hetian Sheep
摘要
Abstract
The aim of this study was to investigate the effects of Oxytropis glabra DC on the content and transcriptional expression of α-mannosidase( AMA) of brain in Hetian sheep,and reveal the toxic mechanism of O.glabra DC.12 Hetian sheep were randomly and equally divided into 3 groups( the control group and exper-imental groupⅠ,Ⅱ) ,the dried plant of O.glabra DC was comminnuted,and the two experimental groups were fed with O.glabra DC at 10 g and 20 g per kg weight everyday respectively during the test period.The sheep consumed the forages freely until typical symptoms were observed.Brain were collected and the contents of AMA and its mRNA expression in different encephalic regions were measured on the 35th day.It was found that AMA in golgi and lysosomal was expressed in brain of all groups, O.glabra DC inhibited the expression of brain AMA1 and AMA2 mRNA,and the difference was obvious.The expression of AMA2 mRNA was not obvious in the brain of experimental groupⅠand the control group( P>0.05) ,the expression of AMA2 mRNA of cerebel-lum in experimental groupⅡwas significantly lower than that in the normal control group(P<0.05),but the expression of AMA2 mRNA was not obvious in experimental groupⅡand the control group in cerebrum,thala-mus,brainstem and hippocampus( P>0.05) .The expression of AMA1 mRNA decreased in experimental groupⅡ,very significant lower than in the control group in cerebellum and thalamus(P<0.01),the expression of AMA1 mRNA decreased in experimental groupⅡ,significant lower than in the control group in cerebrum( P<0.05) ,the expression of AMA1 mRNA of cerebellum in experimental groupⅠwas significantly lower than in the normal control group( P<0.05) ,but the expression of AMA1 mRNA was not obvious in experimental groupⅠand the control group in cerebrum,thalamus,brainstem and hippocampus( P>0.05) .The results showed that O.glabra DC has remarkable effects on the contents of AMA of brain in Hetian sheep.It is indicated that three regions were the effect target sites of O.glabra DC in the brain tissue.It can be concluded that O.glabra DC produces poisoning effect on the contents of AMA and its mRNA expression by promoting cerebellum,cerebrum and thalamus.关键词
小花棘豆/苦马豆素/α-甘露糖苷酶/脑/和田羊Key words
Oxytropis glabra DC/swainsonine/α-mannosidase/brain/Hetian sheep分类
农业科技引用本文复制引用
王帅,贾琦珍,邓利,汪澜,陈根元..小花棘豆中毒对和田羊脑组织α-甘露糖苷酶的影响[J].江西农业大学学报,2016,(1):146-151,197,7.基金项目
国家自然科学基金（31460678）和新疆生产建设兵团博士资金专项（2008JC07）Project supported by the National Natural Science Foundation of China（31460678） and Doctoral Fund of Xin-jiang Production&Construction Corps （）
