口腔颌面外科杂志Issue(6)：412-416,5.DOI:10.3969/j.issn.1005-4979.2015.06.004
Runx2基因转染对兔下颌骨牵张成骨的影响
Runx2 in vivo Gene Therapy Promotes Bone Regeneration in Rabbit Mandibular Distraction Osteogenesis
摘要
Abstract
Objective: To investigate the effects of Runx2 in-vivo gene therapy on new bone regeneration during distrac-tion osteogenesis in rabbit mandibula. Methods: 32 New Zealand maturity male rabbits were established to become the animal model of mandibular distraction osteogenesis, all these rabbit models were divided into experimental (n=16) and control group (n=16) randomly. At the last day of the distraction, Ad5-Runx2 was injected into the distraction gap of ex-perimental group rabbits, while in the control group, injection with the same dose of normal saline. At the end of four weeks after injection, all animals were sacrificed and the distracted mandibles were harvested. Histological, radiological observations, dual energy X ray (DXA) and biomechanical examination were used for evaluation. Results: Three-dimen-sional CT reconstruction images and histological analysis confirmed that the amount of the new bone forming and bone mineral density in the distraction gap of experimental group was significantly higher than the control group. DXA and three-point bending experiment results analysis also showed that the bone mineral density, bone mineral content and maxi-mum load of the distraction gap of experimental group was significantly higher than the control group. Conclusion:Runx2-in vivo gene therapy can effectively promotes the bone regeneration in rabbit mandibular distraction osteogenesis.关键词
Runx2/牵张成骨/骨再生/下颌骨Key words
Runx2/distraction osteogenesis/bone regeneration/mandible分类
医药卫生引用本文复制引用
郑科,宋冬惠,吴森斌,祝颂松,胡静..Runx2基因转染对兔下颌骨牵张成骨的影响[J].口腔颌面外科杂志,2015,(6):412-416,5.基金项目
江苏省“六大人才高峰”项目 （）
