临床小儿外科杂志Issue(1)：29-33,5.DOI:10.3969/j.issn.1671-6353.2016.01.009
P-Smad 3在胆道闭锁肝纤维化中的作用机制研究
The study on mechanism of p-smad3 in hepatic fibrosis of biliary atresia
摘要
Abstract
Objetive To observe the function of TGF—β1 and P—Smad3 etc in TGF—β1 signal pathways in liver fibrosis of biliary atresia.Methods Liver biopsy specimens were collected from congenital biliary dila-tation (CBD group,n =10),biliary atresia patients who had Kasai procedure (Early hepatic fibrosis group,n =19),liver transplantation(transplantation group,n =11),the first two groups were collected from Jan.2010 to Jul.2015 in Tianjin childrens′hospital,the last group was collected from Jan.2013 to Jan.2014 in Tianjin first central hospital.The hematoxylin &eosin staining were used to observe the degree of liver fibrosis of every sin-gle sample,immunohistochemistry were used to observe the expression of TGF — β1、Smad 2 —4、P — Smad 2 ~3 and PAI—1 in liver tissues of these samples.Results HE and Masson:the CBD group had mild fiber cells hy-perplasia,the Kasai group had Proliferation of collagen fibers and bridging fibrosis phenomenon,the transplanta-tion group had significant pseudolobule.Immunohistochemistry:①qualitative analysis:in the CBD group,Smad 2,Smad 4,PAI — 1 expression is positive,the rest is weakly positive;in the Kasai group,the all proteins in the TGF —β1 signal pathways expressed in the liver cytoplasm,P— Smad 2,P — Smad 3,Smad 4 expression was also found in the nucleus;in the liver transplantation group,the expression of all proteins in the TGF — β1 signal pathways were weakly positive.②semi-quantitative analysis;The level of TGF—β1、Smad 3、P—Smad 3 and PAI— 1 in the Kasai group have a significant rise than the other groups(P <0.05),the hepatic fibrosis is becoming serious with the rise of TGF —β1、Smad 3、P—Smad 3 and PAI — 1,at the same time The correlation between P-Smad 3 and PAI — 1 was remarkable.there were no difference of Smad 2、P — Smad 2、Smad 4 between these groups(P >0.05).All of the proteins in the TGF —β1 signal pathways were decreased in transplantation group (P <0.05). Conclusions TGF—β1 and Smad 3 made a contribution to liver fibrosis in the early stage of bili-ary atresia,and P—Smad 3 is closely related with the progress of liver fibrosis.关键词
胆道闭锁/转化生长因子 β1/肝/病理学/研究Key words
Biliary Atresia/Transforming Growth Factor betal/Liver/PA/Research引用本文复制引用
丁美云,高婷,卫园园,赵丽,詹江华..P-Smad 3在胆道闭锁肝纤维化中的作用机制研究[J].临床小儿外科杂志,2016,(1):29-33,5.基金项目
1，天津市卫计委重点攻关项目，项目号14KG129，2，天津市卫生局重点项目，项目号2014KR09；3，国家自然科学基金项目，项目号81570471。 （）
