临床小儿外科杂志Issue(1)：34-37,4.DOI:10.3969/j.issn.1671-6353.2016.01.010
HIF-1α和 VEGF 在胆道闭锁患儿肝组织中的表达及意义
The expression of HIF-1αand VEGF in the patients with biliary atresia
摘要
Abstract
Objetive To detect the expression of HIF—1αand VEGF in the liver of patients with biliary atresia(BA)and their relationship with angiogenesis and fibrosis and to explore their function in the pathogene-sis of BA. Methods Liver tissue of fifteen BA patients、ten congenital biliary dilatation patients、ten liver transplantation patients were collected.HE、Vimentin staining were used to observe the histological changes and detect the degree of fibrosis.CD34 staining mark vessel to count microvascular density (MVD).The immuno-histochemistry were used to detect HIF — 1αand VEGF in the liver of each group. Results MVD in CBD group、BA group、LT group respectively was 16.8 ±1.9、23.2 ±3.8、25.9 ±2.1.HIF — 1a express in cytoplasm of liver cells and some expressed in nucleus,BA group express HIF — 1a more than CBD group、LT group,P <0.05.VEGF express in liver cells、bile duct、the arterial wall,the optical density of VEGF of BA group more than CBD group、LT group,P <0.05. Conclusions The expression of HIF—1αinduced by hypoxia may play a role in the process of BA angiogenesis through regulating the expression of VEGF.关键词
胆道闭锁/血管生成/HIF1-α/VEGF/纤维化Key words
Bliliary atresia/angiogenesis/HIF1 -α/VEGF/fibrosis引用本文复制引用
卫园园,丁美云,高婷,胡晓丽,詹江华..HIF-1α和 VEGF 在胆道闭锁患儿肝组织中的表达及意义[J].临床小儿外科杂志,2016,(1):34-37,4.基金项目
1，天津市卫计委重点攻关项目（项目号14KG129）；2，天津市卫生局重点项目（项目号2014KR09）；3，国家自然科学基金 （）
