农业环境科学学报Issue(2)：380-386,7.DOI:10.11654/jaes.2016.02.024
高效纤维素分解菌的分离鉴定及堆肥效果研究
Isolation and identification of a cellulolytic bacterium and its composting application
摘要
Abstract
Composting is a good approach to utilizing agricultural organic wastes. Supplying microorganisms could shorten composting period and improve compost quality. In this study, a cellulose-decomposing strain was isolated from natural composting manure by using congo red staining and CMC-Na plate method. The strain was identified according to morphological observation, biochemical testing and 16S rDNA gene sequence. The optimal fermentation conditions were also determined by using single factor and orthogonal experiment. Finally, the iso-late was inoculated into cattle mature to test its effect. The strain Y2 was identified as Bacillus subtilis;Its FPA and CMCA were 15.83 U· mL-1 and 100.81 U·mL-1, 1.3 times and 2.76 times higher than originals, respectively. Compost inoculated with Y2 and EM microbial agents reached high temperature after 3 d, with high temperature maintained for 9 d and 8 d, respectively. The degradation rate of cellulose was 42%for Y2 group, while it was 35%for EM microbial agent and 10.5%for sterile water. Therefore, strain Y2 has a great potential to be ap-plied to composting fermentation.关键词
纤维素降解菌/筛选鉴定/产酶条件优化/堆肥发酵Key words
cellulose decomposing bacterium/screening and identification/optimized conditions/composting分类
资源环境引用本文复制引用
张喜庆,勾长龙,娄玉杰,徐佳萍,高云航..高效纤维素分解菌的分离鉴定及堆肥效果研究[J].农业环境科学学报,2016,(2):380-386,7.基金项目
国家现代农业产业技术体系建设专项“牛场环境控制与废弃物处理技术”（CARS-38）；2013年公益性行业（农业）科研专项“牛场废弃物处理利用技术模式集成与示范”（201303091）；吉林省青年科研基金项目 （）
