农业环境科学学报Issue(2)：403-408,6.DOI:10.11654/jaes.2016.02.027
蚯蚓体内恩诺沙星残留的检测方法研究
A detection method for enrofloxacin residues in earthworms
摘要
Abstract
An assay method using high performance liquid chromatography(HPLC)with fluorescence detection was established to quantify enrofloxacin residues in earthworms. The extraction solution comprised 0.1 mol·L-1 phosphoric acid(pH12)+acetonitrile(1+3,V/V). The initial extracts were defatted, evaporated, and re-dissolved in mobile phase consisting of 0.025 mol·L-1 phosphoric acid/triethylamine buffer (pH3.0)+acetonitrile(83+17,V/V). The fluorescence detector excitation wavelength was 280 nm, and the emission wavelength 450 nm. The linear range of the standard curve was 5~500 ng·mL-1. The recoveries of 3 concentration groups were between 81%and 88%with an average of 84.5%and the coefficient of variation was less than 10%. The detection limit of enrofloxacin was 2 ng·g-1. Earthworms were ex-posed to filter papers soaked in enrofloxacin with concentrations of 0, 0.16, 1.6, 16 μg·g-1 and 32μg·cm-2, and 4 replicates in each case. After 48 hours of exposure, the average enrofloxacin concentration in earthworms was 0, 1.53, 16.81, 113.32μg·g-1 and 120.83μg·g-1, re-spectively. These results show that the absorption of enrofloxacin by earthworms increased linearly up to 1.6 μg·cm-2 exposure concentra-tion, and then asymptotically to a maximum of approximately 120μg enrofloxacin·g-1 earthworm tissue.关键词
恩诺沙星/蚯蚓/高效液相色谱法/回收率/滤纸染毒Key words
enrofloxacin/earthworm/HPLC/recovery rate/filter paper exposure分类
资源环境引用本文复制引用
丁丽玲,鲁晓旭,李银生,邱江平..蚯蚓体内恩诺沙星残留的检测方法研究[J].农业环境科学学报,2016,(2):403-408,6.基金项目
国家自然科学基金项目 （）
