四川医学Issue(2)：141-143,3.DOI:10.16252/j.cnki.issn1004-0501-2016.02.007
大蒜素通过Wnt信号途径阻遏人宫颈癌细胞周期进展
Allicin Inhibites Cell Cycles of HeLa Cells through Wnt Pathway
汪春燕 1刘玲 1聂丹 1詹平 1陈建琼 1钱燕萍 1毛熙光1
作者信息
- 1. 泸州医学院附属医院妇产科，四川 泸州646000
- 折叠
摘要
Abstract
Objective To investigate the effects of garlicin on the cycle of cervical cancer HeLa cells and its molecular mechanism. Methods HeLa cells cultured in vitro were given different concentrations of garlicin on different time. The effects of garlicin on the proliferation of HeLa cells were measured by WST-8 method,while the effects of garlicin on the cycle of HeLa cells were measured by flow cytometry. After being treated by different concentrations of garlicin for 24 hours,the expression of mRNA protein and β-catenin of HeLa cells were respectively measured by reverse transcriptase polymerase chain reaction ( RT-PCR ) method and Western blot (Western blot) method. Results Garlicin inhibited the proliferation of HeLa cells,and repressed the progress of cell cycle and the expression of key proteinβ-catenin in the cytoplasm. Conclusion Garlicin inhibited the activation of Wnt signal,repressed the expression of key protein β-catenin in the cytoplasm,and thereby prevented the progress of cell cycle and inhibited the proliferation of HeLa cells,which provided a theoretical basis for the development of new drugs to prevent and treat cervical cancer.关键词
大蒜素/细胞增殖/细胞周期/宫颈癌/Wnt/β-cateninKey words
garlicin/cell proliferation/cell cycle/cervical cancer/Wnt/β-catenin分类
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汪春燕,刘玲,聂丹,詹平,陈建琼,钱燕萍,毛熙光..大蒜素通过Wnt信号途径阻遏人宫颈癌细胞周期进展[J].四川医学,2016,(2):141-143,3.
