山东农业大学学报（自然科学版）Issue(1)：9-14,6.DOI:10.3969/j.issn.1000-2324.2016.01.002
核桃炭疽病病原菌鉴定及生物学特性
Identification and Biological Characteristics of Pathogen from Colletotrichum gloeosporioides
摘要
Abstract
Walnut anthracnose disease is a kind of serious disease of walnut all over the world. In this paper, the identification and the biological characteristics of strain TS-09 isolated from the walnut fruits were studied. The results showed that the strain TS-09 was identified as Colletotrichum gloeosporioides based on its morphologic characters, cultural characteristics and four conserved genes(ribosomal DNA-ITS, ACT,β-tub2 and GPDH)sequences analysis and the pathogenicity was powerful. For the growth of strain TS-09, the best medium was PDA. The optimum temperature was 28℃and the optimum pH for mycelium growth was 7.0. The condition of weak acid was favorable for spore production (pH 4.0). During continuous illumination, the mycelium growth was inhibited while the condition of continuous darkness was better for mycelium growth. The mycelium had stronger resistance against temperature and the lethal temperature for mycelium was 60℃(15 min) or 62℃(10 min). Our research expected to offer theory support for prediction and effective control of walnut anthracnose.关键词
核桃炭疽病/鉴定/生物学Key words
Colletotrichum gloeosporioides/identification/biology分类
农业科技引用本文复制引用
王清海,刘幸红,范昆,段春华,牛赡光,吴小芹..核桃炭疽病病原菌鉴定及生物学特性[J].山东农业大学学报（自然科学版）,2016,(1):9-14,6.基金项目
山东省科技发展计划项目:核桃炭疽病新型健康管理模式构建 （）
山东省农业重大应用技术创新课题:生物药剂在冬枣、核桃生产中的选配与应用 （）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
