山东农业大学学报（自然科学版）Issue(1)：19-24,6.DOI:10.3969/j.issn.1000-2324.2016.01.004
香榧转录组测序及生物信息学基础分析
Sequencing and Bioinformatic Analysis for Transcriptome of Torreya grandis Fort. ex Lindl. cv. merrillii
摘要
Abstract
Torreya grandis Fort. ex Lindl. cv. Merrillii is an economically important plant on both agriculture and ecology. However, the genomic information of this species has been less studied, leading to limited researching progresses in both molecular biology and gene functions. In the present study, we have sampled different tissues of T. grandi to conduct a transcriptomic analysis using the Illumina HiSeq™ 2000 technical platform. As a result, a total of 37,349,086 reads were obtained with the whole base number of 4.35 G. Based on the assembling of high quality reads, we identified a total of 104,636 Unigenes with an average length of 784 nt and N50 was 1,702 nt. Comparing these Unigene sequences with those in the public database, 28,766 Unigenes were annotated in the Nr database, 24,003 Unigenes were in the NT database, and 21,401 Unigenes were in the Swiss-Prot database. Moreover, based on the COG database and the GO database, we also found 16,137 Unigenes and 11,410 Unigenes were in both databases respectively. We further classified 18,564 Unigenes into 256 pathways according to the KEGG annotation information. Finally, we identified 4,706 SSR loci in 4,217 Unigenes via SSR loci searching. The obtained transcriptome data was thus as the first genomic-wide database serving for future studies of T. grandis in terms of functional gene cloning, gene expression, fingerprint construction and molecular marker-assisted breeding.关键词
香榧/转录组/高通量测序/SSRKey words
Torreya grandis/transcriptome/Illumina sequencing/SSR分类
生物科学引用本文复制引用
易官美,包燕春..香榧转录组测序及生物信息学基础分析[J].山东农业大学学报（自然科学版）,2016,(1):19-24,6.基金项目
宁波市科技局农业重大专项项目(2014C11006) （2014C11006）
宁波市自然科学基金(2015A610267) （2015A610267）
