山东农业大学学报（自然科学版）Issue(1)：25-29,5.DOI:10.3969/j.issn.1000-2324.2016.01.005
辣椒疫霉病无毒基因RXLR128001克隆与蛋白表达纯化
Clone of Nontoxic Gene RXLR128001 in Phytophthora capsici and Expression and Purification of Effector Protein
摘要
Abstract
In this study, we cloned a nontoxic gene RXLR128001 from Phytophthora capsici and then analyzed the sequence structure of it based on the BLAST and SignalP4.1 Server software. At the same time, we expressed and purified RXLR128001 gene in Escherichia coli and determined the optimal conditions that could induce RXLR128001 gene to be effective expressed. Finally, we prepared the high pure protein of RXLR128001 gene to be beneficial to the further study on the crystal cultivation and optimization of it.关键词
辣椒疫霉/效应蛋白/基因克隆/蛋白表达纯化Key words
Phytophthora capsici/effector protein/gene clone/expression and purification of protein分类
农业科技引用本文复制引用
王晓梅,张钟文,杨楠..辣椒疫霉病无毒基因RXLR128001克隆与蛋白表达纯化[J].山东农业大学学报（自然科学版）,2016,(1):25-29,5.基金项目
转基因生物新品种培育重大专项(2014ZX0801004B) （2014ZX0801004B）
