山东医药2016，Vol.56Issue(8)：10-13,4.DOI:10.3969/j.issn.1002-266X.2016.08.004
非酒精性脂肪肝大鼠肝组织FGF21mRNA表达与脂质沉积、胰岛素抵抗的关系
Correlations of FGF21 mRNA expression with fat deposition and insulin resistance in rat liver with NAFLD
摘要
Abstract
Objective To observe the fibroblast growth factor 21 ( FGF21 ) mRNA expression level in rat liver with non-alcoholic fatty liver disease ( NAFLD ) and to investigate its relationship with fat deposition and insulin resistance . Methods Forty SD male rats were randomly divided into the control group (n=10, NC group) fed with basic food and high-fat group (n=30, HF group) fed with high-fat diet.After 8-week feeding, 10 rats were selected to observe whether the NAFLD models were built successfully in the HF group .After that the rest of rats in the HF group were fed with high-fat diet, and at week 12, 10 rats were selected and at week 24, the other 10 were selected.The fasting blood-glucose (FPG), fasting insulin (FINS), alanine transamianse (ALT), aspartate transamianse (AST), total cholesterol (TC), triglyceride ( TG) , serum FGF21, serum FFA, and homeostasis model assessment of insulin resistance ( HOMA-IR) were calculated.The pathological change of liver was observed by HE staining;liver triglyceride ( TG) content was tested and the expression of FGF21 mRNA in liver was detected by RT-PCR.Results At the 8 th week, the body mass, liver-wet weight, serum TG and TC of the HF group were higher than those of the NC group (all P<0.05), liver tissues of the HF group changed to bullous steatosis and had inflammatory cell infiltration and spotty necrosis , so that NAFLD model was built successfully.In the end of 12 and 24th weeks, the serum FFA, TG content and HOMA-IR were significantly higher than those of the 8th week, and the 24th week was also higher than the 12th week (all P<0.05).During the week 8-12, the serum FGF21 and expression of FGF21 mRNA in liver of the HF group increased first and then decreased (all P<0.05). Conclusion The expression of FGF21 mRNA in liver and serum FGF21 of NAFLD rats were related with the liver TG con-tent.Besides, both of them have the same change in the early stage of NAFLD , and insulin resistance may inhibit the ex-pression of FGF21mRNA indirectly.关键词
非酒精性脂肪性肝病/成纤维细胞生长因子/脂质沉积/胰岛素抵抗/大鼠Key words
non-alcoholic fatty liver disease/fibroblast growth factors/lipidoses/insulin resistance/rat分类
医药卫生引用本文复制引用
申甜,徐碧林,盛霞,高月锦,汪红平,喻明..非酒精性脂肪肝大鼠肝组织FGF21mRNA表达与脂质沉积、胰岛素抵抗的关系[J].山东医药,2016,56(8):10-13,4.基金项目
上海市卫生局青年科研项目(2013Y079) （2013Y079）
上海市中医药大学后备业务专家培养计划. （）
