山东医药2016，Vol.56Issue(8)：14-16,后插1,4.DOI:10.3969/j.issn.1002-266X.2016.08.005
miR-34a在结肠癌细胞增殖和迁移中的作用及其分子机制
Function and molecular mechanism of miR-34 a in proliferation and migration of colon cancer
摘要
Abstract
Objective To explore the role of miR-34a in the proliferation and migration of colon cancer cells , and to validate the target protein .Methods The empty vector ( pRI-CMV-GFP) and lentiviral vector ( pRI-CMV-GFP-miRNA-34a vector) were transfected into human colon cancer cell line ( HCT-116) as the negative control group and lentiviral group, while HCT116 cells without any processing were regarded as the blank control group .The expression of miR-34a was detected by real-time PCR.MTT and Transwell assays were used to detect HCT-116 cell proliferation and migration . Immunofluorescence and Western blotting were applied to verify the target gene of miR-34a in HCT-116.Results The ex-pression of miR-34a in the blank control group was taken as 1, the expression of miR-34a in the negative control group and lentiviral group was 1.03 ±0.09 and 6.41 ±1.56, respectively.The lentiviral group was higher than the negative control group and blank control group (all P<0.01), which confirmed lentiviral transfection was successful .Compared with the blank control group and negative control group , the proliferation and migration abilities of HCT-116 cells in the lentiviral group were significantly decreased (all P<0.01).Moreover, the fluorescence intensity of c-Met in the lentiviral group was significantly decreased while no change was found in the other two groups .Western blotting showed that the expression of c-Met and phosphorylated c-Met in the lentiviral group was lower than that of the negative control and blank control group ( all P<0.01).Conclusion Over-expression of miR-34a inhibits the proliferation and migration of colon cancer cells and down-regulates the target protein c-Met and phosphorylated c-Met expression, which suggests miR-34a could be a effective molecular target in treatment of colon cancer.关键词
结肠癌/miR-34a/细胞增殖/细胞迁移/c-MetKey words
colon cancer/miR-34a/cell proliferation/cell migration/c-Met分类
医药卫生引用本文复制引用
庄彪,闵志均,王延峰,张鹏,倪熊,瞿惠龙,丁育明..miR-34a在结肠癌细胞增殖和迁移中的作用及其分子机制[J].山东医药,2016,56(8):14-16,后插1,4.基金项目
上海市浦东新区卫生和计划生育委员会卫生科技项目(PW2014A-28). （PW2014A-28）
