山东医药2016，Vol.56Issue(8)：20-22,后插2,4.DOI:10.3969/j.issn.1002-266X.2016.08.007
苯基乙酰对小鼠移植性肺癌的抑制作用及其机制
Inhibitory effect of phenylacetylglutamine on metastatic lung caner of mice
摘要
Abstract
Objective To observe the inhibitory effect of phenylacetylglutamine ( PG) on lung caner and to investigate the mechanism.Methods The lung cancer metastasis models in C57BL/6 mice was generated by intravenous injection of Lewis lung carcinoma ( LLC) cells.Then the model mice were randomly divided into PG group ( n=20) which was injec-ted with 800 mg/kg PG and PBS group (n=20) which was injected with the same amount of PBS , both for 10 days.We randomly took 5 mice in each group to calculate the tumor count and measure the size of tumor .We randomly took 5 mice in each group to detect the expression of Bcl-2, Bcl-XL, cIAP1, Survivin and PCNA proteins by Western blotting .The broncho alveolar lavage fluid ( BALF ) from 5 mice were collected to detect inflammatory cells and inflammatory factors (TNF-α, IL-6, KC).The electrophoretic mobility shift assay (EMSA) was used to detect the NF-κB DNA binding abili-ty.Results Compared with the PBS group , the growth of lung tumors in mice was inhibited , and tumor number and tumor size decreased significantly in the PG group (P<0.05).The expression of Bcl-2, Bcl-XL, cIAP1, Survivin and PCNA was significantly decreased in the PG group .In BALF of the PG group, binding activity between NF-κB and target DNA was strongly suppressed .Compared with the PBS group , the number of BALF of various inflammatory cells and inflammato-ry cytokines were significantly reduced in the PG group (all P<0.05).Conclusion PG can inhibit the development of lung tumor, and the mechanism may be related with inducing lung tumor cell apoptosis , inhibiting the activation of NF-κB, and reducing the inflammatory reaction.关键词
肺癌/苯基乙酰/巨噬细胞/核因子-κB/炎症反应/小鼠Key words
lung cancer/phenylacetylglutamine/macrophage/nuclear factor-kappa B/inflammation reaction/mice分类
医药卫生引用本文复制引用
权文强,吴军录,姚懿雯,万海英,李冬..苯基乙酰对小鼠移植性肺癌的抑制作用及其机制[J].山东医药,2016,56(8):20-22,后插2,4.基金项目
国家自然科学基金资助项目(81272603,81472179) （81272603,81472179）
上海市浦江人才计划(13PJ1407300) （13PJ1407300）
上海申康医院发展中心市级医院临床辅助科室能力建设项目(SHDC22014008). （SHDC22014008）
