上海农业学报Issue(1)：61-65,5.DOI:10.15955/j.issn1000-3924.2016.01.14
分光光度法测定灵芝中总三萜含量方法探讨
Discussion on the determination of total triterpenoids in Ganoderma lucidum by spectrophotometry
摘要
Abstract
Total triterpenoid content in fruit body,mycelium and spore powder of Ganoderma lucidum were determined by spectrophotometry and HPLC respectively,which showed that total triterpenoid content measured by different methods was quite different.The color reaction results of vanillin perchloric acid with different Gano-derma lucidum acid standard and oleic acid,ergosterol showed that the optimal absorption wavelengths of different Ganoderma lucidum acids are 530—550 nm.The measured value at 550 nm was quite different,especially the determination values of Ganoderma lucidum acid A,B and C with high content were seriously low,and the interfering substances of oleic acid and ergosterol had serious interference on the determination value.The results show that the determination value of total triterpene content in Ganoderma lucidum by spectrophotometry could not accurately reveal the actual total triterpene content of Ganoderma lucidum,which was not suitable for the determination of total triterpene content of Ganoderma lucidum.关键词
灵芝/三萜/分光光度法/HPLCKey words
Ganoderma lucidum/Triterpenoids/Spectrophotometry/HPLC分类
农业科技引用本文复制引用
张忠,张劲松,刘艳芳,周帅,王金艳,于华峥,唐庆九..分光光度法测定灵芝中总三萜含量方法探讨[J].上海农业学报,2016,(1):61-65,5.基金项目
国家科技支撑计划子课题［食用菌功能因子提取制备及产品开发与产业化（2013BAD16B08-02）］；公益性行业（农业）专项子课题［食用菌副产物中活性物质提取及富集技术研究与示范（201303080）］ （）
