上海农业学报Issue(1)：66-71,6.DOI:10.15955/j.issn1000-3924.2016.01.15
高产洛蒙真菌素的洛蒙德链霉菌 S015的诱变筛选
Induced mutation and selection of Streptomyces lomondensis S015 producing high lomofungin yield
摘要
Abstract
A mutant strain S01 5-P34 was selected from a wild lomofungin-producing Streptomyces lomonden-sis strain S01 5 through mutagenesis induced by phenzine-1 -catboxylic acid (PCA),and its lomofungin yield reached(1 06.0 ±4.4)mg?L and 3.2 times of the wild strain’s;Mutant strains S01 5-R6 and S01 5-G1 1 were se-lected from the strain S01 5 through mutagenesis induced by rifampicin and gentamicin antibiotics,and their lomo-fungin yields were respectively(1 03.4 ±0.1 )mg?L and(81 .2 ±2.5)mg?L and respectively 3.2 times and 2.5 times of the wild strain’s;The PCA-induced mutagenesis was combined with the rifampicin-induced mutagenesis and a mutant strain S01 5-R6-46 was obtained,with the lomofungin yield being(1 68.0 ±2.2)mg?L and 5.1 times of the wild strain’s.The optimization of S01 5-R6-46 culture medium showed that the lomofungin yield could reach(258.7 ±5.7)mg?L via addition of 0.1 mmol?L FeCl3 .The above experimental results could be used for reference in induced mutation and selection of streptomycete strains producing high phenazine yields.关键词
洛蒙德链霉菌/诱变/筛选/洛蒙真菌素/吩嗪类化合物Key words
Streptomyces lomondensis/Induced mutation/Screening/Lomofungin/Phenazine compounds分类
农业科技引用本文复制引用
张春晓,钱玮,王威,胡洪波,彭华松,张雪洪..高产洛蒙真菌素的洛蒙德链霉菌 S015的诱变筛选[J].上海农业学报,2016,(1):66-71,6.基金项目
国家自然科学基金（31270084）；国家973计划项目（2012CB721005）；国家863计划项目 （）
