中国生态农业学报Issue(3)：284-292,9.DOI:10.13930/j.cnki.cjea.150859
三七收获后不同年限土壤微生物代谢多样性分析
Soil microbial diversity analysis after different years of Panax notoginseng harvest
摘要
Abstract
Continuous cropping obstacle is a key limit factor of Panax notoginseng cultivation. The reason for continuous cropping obstacles, such as changes of soil microorganisms have not been clearly, especially under natural conditions. To evaluate the impact of P. notoginseng planting on soil microbial communities and continuous cropping obstacle mechanisms, the soil nutrients and soil microorganism community structure were investigated in different fields after different years of P. notoginseng harvested in three towns (Ganhe, A’meng and Panlong) in Wenshan City, Yunnan Province. In each town, 7 fields were selected as soil sampling sites. Seven fields were planted other corps rather than P. notoginseng, six of which were at 1 to 6 years after P. notoginseng harvested, the other one which never planted P. notoginseng was set as the control (CK). Biolog-ECO method was used to analyse the average well color development (AWCD) and diversity of microbial community to explore microbial community dynamics. The results showed that AWCD of soil microorganisms was not regularly changed with increasing years after P. notoginseng harvested. After cultivated for 96 hours, activity of soil microorganisms reached the highest level. Cluster analysis of utilization of 6 groups (31 kinds) of carbon resources showed that in three towns, carbon resources utilization of soils at 1 and 6 years after P. notoginseng harvested were classed in one group, indicting the same characteristics of carbon resources utilization. No obvious regularity was observed for other years. The use of carbohydrates, polymeric substances, carboxylic acid and phenols by soil microorganisms in 1 and 6 years after P. notoginseng harvested were 25.97%-55.59% and 53.14%-65.68% more than those of CK. Soil nitrogen, phosphorus, potassium and organic matter contents increased at 2 years, decreased at 4 and 5 years after P. notoginseng harvested. Soil nitrogen, phosphorus, potassium and organic matter contents in three towns, as well as Shannon-Wiener index, evenness index and Simposon’s index of soil microorganisms in A’meng and Panlong Towns at 1 to 6 years after P. notoginseng harvested were not significantly different compared with that of CK. The results suggested that rotation with other crops at least for 6 years was indispensable to avoid continuous cropping obstacles of P. notoginseng.关键词
三七/连作障碍/微生物代谢/Biolog-ECO/功能多样性/多样性指数Key words
Panax notoginseng/Continuous cropping obstacle/Microbial metabolism/Biolog ECO/Functional diversity/Diversity index分类
农业科技引用本文复制引用
靳百慧,何霞红,杨羚钰,徐玉龙,张义杰,魏正孟,康洪宽,侯永明,朱书生,朱有勇..三七收获后不同年限土壤微生物代谢多样性分析[J].中国生态农业学报,2016,(3):284-292,9.基金项目
云南省发展和改革委员会重大专项项目(20112513)资助* The study was supported by the Special Project of Development and Reform Commission in Yunnan Province (20112513) （20112513）
