生物技术通报Issue(1)：103-108,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.017
甘蔗一次多基因遗传转化及多重 PCR 检测
Multiple Gene Transformation in Sugarcane and Multiple PCR Detection
摘要
Abstract
Multiple traits of crops such as rice and corn had been improved in single time of transformation by the strategy of multiple gene transformation. For exploring the feasibility of multiple gene transformation of sugarcane in this research, a multiple gene expression vector was used and transformed to sugarcane and many transformed plants were produced. Multiple PCR method was used to analyze the integration and deficiency of multiple genes in the transformed plants. Results showed that multiple gene transformation of sugarcane was feasible. But due to the insertion of large DNA fragments some genes were lost during the integration. Thus it is a need to have sufficient transformed plants then valuable transformed plants can be selected for further research.关键词
甘蔗/农杆菌介导法/多基因遗传转化/多重PCR检测Key words
sugarcane/Agrobacterium-mediated/multiple gene transformation/multiple PCR detection引用本文复制引用
王文治,杨本鹏,蔡文伟,熊国如,王俊刚,冯翠莲,张树珍..甘蔗一次多基因遗传转化及多重 PCR 检测[J].生物技术通报,2016,(1):103-108,6.基金项目
国家自然科学基金项目（31371687），现代农业产业技术体系建设专项资金项目 （）
