生物技术通报Issue(1)：109-114,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.019
山葡萄 CBF 基因表达载体构建及对拟南芥根生长的影响
The Construction of Expression Vector of Gene CBF from Vitis amurensis and the Effects of It on the Root Growth of Arabidopsis
摘要
Abstract
In order to investigate the response mechanism of CBF gene in the regulation of plant to the salt stress, the plant over-expression vectors of VaCBF1, VaCBF2 and VaCBF3 from Vitis amurensis were constructed. The results by restriction enzyme digestion and agarose gel electrophoresis showed that 3 genes were correctly inserted into pBASTA, indicating that the expression vectors were successfully constructed. Then, 3 over-expressed vectors were transformed into Agrobacterimu tumefaciens EHA105, and Arabidopsis thaliana was leached using floral dip method. The transgenic plants of A. thaliana with over-expression of 3 genes were screened by herbicide Basta. Furthermore, wild and transgenic plants were treated with salt stress. The results revealed that the elongation length of primary root of OE-CBF2 transgenic plant was longer than others, and the lateral roots in 3 genes transgenic plants were longer than wild ones. These results indicate that the CBF genes of V. amurensis play the critical regulation role in the root development during salt stress.关键词
山葡萄/CBF基因/表达载体/根生长Key words
Vitis amurensis/CBF gene/expression vector/root growth引用本文复制引用
王法微,董金晔,李晓薇,刘洋,吴学彦,王南,董园园,陈欢,李海燕..山葡萄 CBF 基因表达载体构建及对拟南芥根生长的影响[J].生物技术通报,2016,(1):109-114,6.基金项目
国家自然科学基金项目（31201144，31271746），吉林省发改委项目（JF2012C002-04），吉林农业大学国家大学生创新创业训练计划项目 （）
