生物技术通报Issue(1)：115-123,9.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.020
日本结缕草‘胶东青’DREB2.2基因克隆及表达模式研究
Cloning and Expression Profile of DREB2.2 Gene from Zoysia japonica var. pallida cv Jiaodong
摘要
Abstract
DREB(dehydration responsive element binding protein)is a type of transcription factor commonly existing in higher plants, and involves in abiotic stress response and the process of growth and development. In this study, using Zoysia japonica var. pallida cv Jiaodong as material, the coding sequence of gene DREB2.2 was cloned, then the bioinformatics were analyzed, and the expression profile of the gene in the stress environment was detected by semi-quantitative PCR technique. Sequencing analysis indicated that DREB2.2 had 2 transcripts of long and short. The long transcript, DREB2.2-L, was 1 067 bp in length, with a premature ORF because of a 50 bp insertion sequence, and putatively encoding 65 amino acids. The short one, DREB2.2-S, was 1 017 bp in length, encoding 338 amino acids ;the putative protein was 37.3 kD, pI was 4.86, and it belonged to the A-2 group of DREB subfamily, containing an AP2 conservative structure domain and nuclear localization sequence. The results of semi-quantitative PCR showed that both DREB2.2-S and DREB2.2-L were expressed in normal condition, the expressions were up-regulated under low temperature stress with the highest level at 2 h exposure, and were slightly up-regulated during the 2 h and 24 h of drought stress, but showed no response to high salt stress. In both normal and stress conditions, the mRNA amount of DREB2.2-L was slightly higher than that of DREB2.2-S.关键词
日本结缕草‘胶东青’/转录因子/DREB/非生物逆境Key words
Zoysia japonica var. pallida cv Jiaodong/transcription factor/DREB/abiotic stress引用本文复制引用
可祥,农钧琇,石大林,马礼鹏,李京,韦善君..日本结缕草‘胶东青’DREB2.2基因克隆及表达模式研究[J].生物技术通报,2016,(1):115-123,9.基金项目
国家自然科学基金项目（31100507），中央民族大学大学生创新训练项目 （）
