生物技术通报Issue(1)：144-148,5.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.024
猪γ干扰素在家蚕中的表达和抗病毒活性检测
Expression and Antiviral Activity Detection of Porcine Interferon-gamma in Silkworm
摘要
Abstract
Interferon is used for threating viral infectious diseases and enhancing vaccine efficacy in stockbreeding. In this study, recombinant porcine interferon-gamma(PoIFNγ)was expressed in silkworm by using Baculovirus expression vector system. Based on the published sequences, the gene of PoIFNγ was artificially synthesized after codon optimization. The optimized gene was firstly cloned into pVL1393 transfer vector of Baculovirus, with virus BmBacmid DNA which was co-transfected into BmN cell line, and recombinant BmNPV was obtained. The PoIFNγ gene was in the downstream of polyhedrosis gene promoter in BmNPV. The silkworm larva infected with recombinant BmNPV gained the expression of PoIFNγ that was detected by Western blotting. The activity of interferon was measured by using micro-cytopathic method that uses interferon to inhibit the VSV-GFP infecting VERO cells. The results showed that the titer of interferon was over 6×105 IU/mL. The activated PoIFNγ was successfully expressed in silkworm larvae.关键词
猪γ干扰素/家蚕/杆状病毒表达系统/抗病毒活性Key words
porcine interferon-gamma/silkworm/Baculovirus expression system/antiviral activity引用本文复制引用
刘兴健,李皓洋,胡小元,张志芳,易咏竹,李轶女..猪γ干扰素在家蚕中的表达和抗病毒活性检测[J].生物技术通报,2016,(1):144-148,5.基金项目
国家高技术研究发展计划“863”（2011AA100603），国家重点基础研究发展计划 （）
