生物技术通报Issue(1)：163-168,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.027
斑马鱼 SFPQ 蛋白的原核表达及纯化
Prokaryotic Expression and Purification of Zebrafish SFPQ
杨川 1胡敏1
作者信息
- 1. 四川大学生命科学学院，成都 610041
- 折叠
摘要
Abstract
Splicing Factor Proline and Glutamine Rich(SFPQ)is a protein that expresses widely in vertebrates. As a tumor suppressor gene, it plays the vital regulation role in tumorigenesis of living organisms. So far the researches of SFPQ were focused on human being and mice, little has been known about the SFPQ of zebrafish. In order to acquire the prokaryotic-expressed SFPQ protein for further research, recombinant pET28a-SFPQ plasmid of zebrafish was constructed and transformed into the chemical competent cells of BL21. The optimal condition was explored while IPTG was applied to induce the expression of zebrafish SFPQ protein in low-temperature, and the SFPQ protein was purified by Ni-NTA agarose nickel column. The results demonstrated that the expression of the protein was the most while inducing for 10 h by 0.75 mmol/L IPTG at 27℃. The results of Western blot revealed that the purified SFPQ protein specifically bound with the antibody, indicating that the purified zebrafish SFPQ protein may be utilized in the further experiments.关键词
斑马鱼SFPQ/原核表达/蛋白纯化Key words
zebrafish SFPQ/prokaryotic expression/protein purification引用本文复制引用
杨川,胡敏..斑马鱼 SFPQ 蛋白的原核表达及纯化[J].生物技术通报,2016,(1):163-168,6.
