生物技术通报Issue(1)：207-212,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.01.033
复合红景天苷支架材料对大鼠骨髓间充质干细胞的影响
The Effect of Scaffold Combined with Salidroside on Rat Bone Mesenchymal Stem Cells
摘要
Abstract
The objective of this work is to investigate the effect of collagen scaffolds combined with salidroside microspheres on rat bone mesenchymal stem cells(rBMSCs). The salidroside was made into microspheres that combined into the collagen. The surface character of material was observed by scanning electron microscopy(SEM). The material was implanted with rBMSCs and observed for the cell adhesion on the material by SEM. The cell proliferation on the material was measured by CCK-8, and the cell proliferation and morphology on the material were detected by HE staining. S100 immunofluorescence was used to detect the differentiation of stem cells into nerve cells. Cells were seeded onto the scaffold, adhered and grew well on materials. While the time increased, the cells on the same material were significant proliferation(P< 0.05). The expression of nerve cell marker protein S100 was positive. Conclusively, collagen combined with salidroside microspheres had good biocompatibility, salidroside microspheres did not affect cell proliferation on the material, and it could induce effectively rBMSCs into nerve cells.关键词
红景天苷/胶原蛋白/材料支架/骨髓间充质干细胞Key words
salidroside/collagen/scaffold/bone marrow mesenchymal stem cells引用本文复制引用
王九娜,向大伟,唐俊杰,李根,赵玲,秦文,赵红斌..复合红景天苷支架材料对大鼠骨髓间充质干细胞的影响[J].生物技术通报,2016,(1):207-212,6.基金项目
国家自然科学基金面上项目 （）
