现代检验医学杂志Issue(1)：112-114,3.DOI:10.3969/j.issn.1671-7414.2016.01.032
异种去细胞角膜基质浸提液对 CHL 细胞毒性的实验研究
Research on the Cytotoxicity of the Heterogeneous Acellular Corneal Stromal Leaching Solution on the CHL Cells
摘要
Abstract
Objective To select the largest non-toxic leaching solution concentration through the experimental observation of the cytotoxicity of the ostrich acellular corneal stromal leaching solution to the Chinese hamster lung fibroblasts cells(CHL) for the further chromosome distortion experiment.Methods The leaching solution made from the ostrich acellular corneal stromal material was diluted with concentrate of 1 ∶ 2,1 ∶ 4 and the original concentration were used to culture with the CHL cells,the negative and positive control were also set up at the same time,to evaluate the impact on cell growth after 24 hour by MTT colorimetric method.Results The leaching solution diluted with 1∶4 was non-toxic,and could promote the growth of the cells.Conclusion Combined with the results of classification and cell morphological features,this cytotoxicity test can be used to screen the best benchmark non-toxic concentrations for the chromosome aberration test of the CHL cells.关键词
鸵鸟去细胞角膜基质浸提液/细胞毒性试验/基准无毒浓度Key words
ostrich acellular corneal stroma/leaching solution/cytotoxicity test/the benchmark non-toxic concentration分类
医药卫生引用本文复制引用
刘先宁,汪耀,肖湘华,潘士印,银勇,安娜,朱秀萍..异种去细胞角膜基质浸提液对 CHL 细胞毒性的实验研究[J].现代检验医学杂志,2016,(1):112-114,3.基金项目
陕西省科技统筹创新工程计划项目（2012KTCQ03-11）。 （）
