物理化学学报Issue(2)：565-572,8.DOI:10.3866/PKU.WHXB201511301
BSA与羟磷灰石相互吸附的FTIR-ATR光谱
FTIR-ATR Spectrometry of BSA Adsorption on Hydroxyapatite
摘要
Abstract
The microcosmic process of bovine serum albumin (BSA) adsorbing onto hydroxyapatite (HA) for different time intervals was investigated by Fourier transform infrared attenuated total internal reflectance (FTIR-ATR) spectrometry. The initial dissolution and re-precipitation of PO43-, Ca2+, and OH-ions from the HA coating led to the occurrence of the coating including adsorbed BSA on the HA from surface-to subsurface-molecular layers and to in-depth interaction between BSA and HA. The subtraction results gained in the adsorption regions of HA and BSA reveal that the binding of P=O, from the phosphate (PO43-), to the hydrogen of amide II, methyl and methene of the BSA appears to be considerably more rapid and stronger than that of the P―O group. In addition, it is very likely that Ca2+plays an important role in the interaction of BSA with HA. It appears that the binding of Ca2+to the carbonyl-oxygen of the peptide bond in BSA caused a significant, molecular, conformational rearrangement of polypeptide backbones fromβ-pleated sheet to helical circles ofα-helix andβ-turn. This change appears to have been fol owed by much hydrogen of polypeptides being driven to bind PO43-and OH-effectively and much―C=O and H―N―groups of the peptide bond being freed from inter-chain hydrogen-bonding to act on Ca2+and combine strongly with the HA surface. This might reasonably be expected to promote hard tissue regeneration. BSA seems to be activated by the inductive effect of Ca2 + via the molecular rearrangement of polypeptide backbones from pleated sheet to helical circles and in turn reacts strongly on the HA, resulting in profound effects on the course of biomineralization.关键词
生物材料/蛋白质吸附作用/多肽/红外光谱/FTIR-ATR/生物矿化作用Key words
Biomaterial/Protein adsorption/Polypeptide/IR spectroscopy/FTIR-ATR/Biomineralization分类
化学化工引用本文复制引用
叶青,胡仁,周剑章,叶艺文,许朝曦,林昌健,林种玉..BSA与羟磷灰石相互吸附的FTIR-ATR光谱[J].物理化学学报,2016,(2):565-572,8.基金项目
The project was supported by the National Natural Science Foundation of China (51571169).国家自然科学基金(51571169)资助项目 （51571169）
