西安交通大学学报（医学版）Issue(2)：204-208,225,6.DOI:10.7652/jdyxb201602012
苯妥英钠对大鼠骨髓间充质干细胞与血管内皮细胞 VEGF 和 SCF 分泌的影响
Effects of phenytoin on VEGF and SCF in rat bone marrow mesenchymal stem cells and vascular endothelial cells co-culture system
摘要
Abstract
ABSTRACT:Objective To investigate the effects of phenytoin (PHT)on the secretion of vascular endothelial growth factor (VEGF)and stem cell factor (SCF)based on the establishment of indirect co-culture system of rat bone marrow mesenchymal stem cells (BMSCs)and vascular endothelial cells (VECs).Methods Indirect co-culture model of rat BMSCs and VECs was established.Experimental groups:indirect co-culture groups (PHT concentrations were 0,20 and 40 μg/mL);the control group:BMSCs culture group and VECs culture group (PHT concentrations were 0,20 and 40μg/mL).The contents of VEGF and SCF in the culture supernatant were measured using double antibody sandwich ABC-ELISA method on cultivation days 2,4,6.Results ELISA assay of the rBMSCs and rVECs in indirect co-culture supernatants,collected on culture days 2,4 and 6 showed that:① VEGF:On culture day 2,VEGF level in the co-culture groups was significantly higher than those in BMSCs group (P <0.05)and rVECs group (P <0.001).As culture time prolonged and PHT concentration increased to 20 μg/mL and 40 μg/mL,VEGF level increased too (P <0.001,P <0.05 ).② SCF testing results showed that the secretion of SCF in co-culture groups was higher than that in the control groups.When PHT was 20 μg/mL,the secretion of <br> SCF increased as the incubation time increased;but as the incubation time increased, PHT concentration of 40 μg/mL made SCF content decrease.Each group did not significantly differ (P > 0.05 ).Conclusion PHT promotes the secretion of VEGF and may reduce the secretion of SCF.关键词
大鼠/骨髓间充质干细胞/血管内皮细胞/间接共培养/血管新生/苯妥英钠Key words
rat/bone marrow mesenchymal stem cells (rBMSCs)/vascular endothelial cells (rVECs)/indirect co-culture/angiogenesis/phenytoin (PHT)分类
医药卫生引用本文复制引用
何珊,赵俪月,巴晓晔,王宝彦,宋建玲..苯妥英钠对大鼠骨髓间充质干细胞与血管内皮细胞 VEGF 和 SCF 分泌的影响[J].西安交通大学学报（医学版）,2016,(2):204-208,225,6.基金项目
陕西省科技厅社发攻关资助项目(No.2012SF2-22) Supported by the Social Development Project of Science and Technology Department of Shaanxi Province (No.2012SF2-22) （No.2012SF2-22）
