西安交通大学学报（医学版）Issue(2)：226-229,4.DOI:10.7652/jdyxb201602016
异丙酚通过激活 PI3K/Akt 信号通路抑制癌细胞转移
Propofol inhibits the metastasis of tumor cells via activating PI3K/Akt signaling pathway
摘要
Abstract
ABSTRACT:Objective To study the effects of propofol on the metastasis of tumor cells related PI3K/Akt signaling pathway.Methods The breast cancer model was established by transplanting human derived breast cancer cell lines into immunodeficient mice with naked gene.The mice,inoculated successfully,were randomly divided into 4 groups:control group (C group,n =6),propofol group (P group,n =6),propofol+PI3K inhibitor (BYL71 9)group (P+B group,n =6),and PI3K inhibitor group (BYL71 9)(B group,n =6).The expressions of PI3K,p-Akt and Akt were examined by Western blot at week 4 after administration;the gene levels of PI3KR1, Akt1 and Akt2 were detected by RT-PCR at week 4 after administration;the number of metastatic lung nodules from both lungs was also observed at week 4 after administration.Results Compared with those in C group,the expressions of PI3K and p-Akt were significantly higher in P group (P <0.05),the level of PI3KR1 mRNA but not Akt1 and Akt2 mRNA was significantly increased(P < 0.05 ),and metastatic lung nodules significantly decreased (P <0.05).In B group,the expressions of PI3K and p-Akt were significantly decreased (P <0.05 ),the levels of PI3KR1,Akt1 and Akt2 mRNA were not significantly increased (P >0.05),but metastatic lung nodules significantly increased (P < 0.05 ).Compared with those in B group,in P+ B group the expressions of PI3K and p-Akt were markedly higher (P <0.05),the level of PI3KR1 mRNA but not Akt1 and Akt2 mRNA was significantly increased (P <0.05),and metastatic lung nodules significantly decreased (P <0.05).Conclusion Propofol can inhibit the metastasis of tumor cells through the upregulated and activated PI3K/Akt signaling pathway.关键词
异丙酚/PI3K/Akt/乳腺癌/转移Key words
propofol/PI3K/Akt/breast cancer/metastasis分类
医药卫生引用本文复制引用
陈军,赵文晖,刘玲玲,谢克亮,宋张骏..异丙酚通过激活 PI3K/Akt 信号通路抑制癌细胞转移[J].西安交通大学学报（医学版）,2016,(2):226-229,4.基金项目
国家自然科学基金资助项目(No.81101409) Supported by the National Natural Science Foundation of China (No.81101409) （No.81101409）
