西安交通大学学报（医学版）Issue(2)：239-243,272,6.DOI:10.7652/jdyxb201602019
PTEN 通过拮抗 PI3K/Akt 信号通路抑制神经干细胞增殖
PTEN inhibits the proliferation of neural stem cells via the antagonism of PI3K/Akt signaling pathway
摘要
Abstract
ABSTRACT:Objective To explore the role of PTEN in the suppression of neural stem cells so as to clarify whether neural stem cell proliferation can be promoted by regulating the PI3K-Akt/PTEN expression level. Methods We removed the hippocampus from neonatal 24 h Kunming mice,isolated and cultured the generation of neural stem cells,which were then identified by immunofluorescence test.We randomly grouped the cultured neural stem cells into normal group,ischemia model group,hypoxia group (hypoxia + ischemia model group),Lip2000 group (hypoxia+ ischemia model group + Lip2000 null transfection group,PTEN transfection group (hypoxia +ischemia model group+Ad5-PTEN transfection group),and PTEN interference group (hypoxia+ ischemia model group+Lip2000+PTENsiRNA interference group).We detected the proliferation of neural stem cells in the groups at different time points,and PTEN protein expression of neural stem cells in each group after PTEN transfection, <br> and the effect on iconic protein on Akt-PI3K signaling pathway.Results (1 )Nestin identification of the neural stem cells was tested by immunofluorescence.We observed green bright and typical cell spheroids under fluorescence microscope;the clear structure could be seen within spheroids.(2)We determined the proliferation of neural stem cells at different time points by MTT.After 36 h of culture,the neural stem cells had obvious proliferation in the hypoxia group and Lip2000 group compared with the model group, the PTEN transfection group and PTEN interference group (P <0.05),including the cell proliferation of PTEN interference group compared to that of the model group,and PTEN transfection group obviously,the differences were also significant (P < 0.05 );(3 ) Compared with the cells in the normal group,PTEN expression increased in the model group and PTEN gene transfection group (P <0.05);it was all lower in other groups than in the normal group (P <0.05).The trend of decrease was similar in hypoxia group,Lip2000 group,and PTEN interference group (P >0.05);(4)After PTEN plasmid transfection,there were no apparent changes in the total Akt and bad in the groups,but compared with the normal group,PI3K,p-Akt,and p-bad expressions in model group and PTEN gene transfection group reduced (P <0.05),with the more obvious changes in the PTEN transfection group.PI3K,p-Akt,and p-bad expressions in the hypoxia group, Lip2000 group and PTEN interference group increased (P < 0.05 ). Conclusion Hypoxia contributes to promoting the proliferation of neural stem cells,and the inhibition of PTEN on PI3K/Akt might be the key factor for blocked proliferation of adult neural stem cells.关键词
神经干细胞/PTEN/PI3K/Akt 信号通路/低氧/bad/海马Key words
neural stem cell/PTEN/PI3K/Akt signaling pathway/hypoxia/bad/hippocampus分类
医药卫生引用本文复制引用
崔晓萍,陈建梅,穆军山,叶建新,林敏,王魁花,林航..PTEN 通过拮抗 PI3K/Akt 信号通路抑制神经干细胞增殖[J].西安交通大学学报（医学版）,2016,(2):239-243,272,6.基金项目
南京军区医药卫生科研基金资助(No.12MA097) Supported by Nanjing Military Command Medical Scientific Research Funds (No.12MA097) （No.12MA097）
