西安交通大学学报（医学版）Issue(2)：279-282,306,5.DOI:10.7652/jdyxb201602027
白花蛇舌草总黄酮对TGF-β1诱导的肝癌MHCC97-H细胞EMT的逆转作用及其机制
Effect and mechanism of total flavones of oldenlendia diffusa willd on epithelial-mesenchymal transition of cell line MHCC97-H induced by TGF-β1
摘要
Abstract
ABSTRACT:Objective To investigate the effects of total flavones of oldenlandia diffusa (FOD)on epithelial-mesenchymal transition in hepatocellular cancer cell line MHCC97-H.Methods TGF-β1 induced EMT in routinely cultured liver cancer cell line MHCC97-H;then MHCC97-H cell was divided into 5 groups:normal control group, TGF-β1 group,TGF-β1 + FOD group,TGF-β1 + 5-FU group,and TGF-β1 + FOD + 5-FU group.After 48 h of treatment,the invasion ability of MHCC97-H cell was detected by Transwell;the proteins of E-cadherin and vimentin were determined by Western blot.Results Compared with the normal form of MHCC97-H cell line,the cell had obvious long fusiform after TGF-β1 induction,and the invasion ability enhanced (P = 0.02 ).But after treatment,the invasion ability of MHCC97-H cell decreased in FOD group and 5-FU group compared with that in TGF-β1 group (P = 0.03,P = 0.02 ),and decreased more significantly in FOD + 5-FU group (P = 0.01 ).The expression of E-cadherin at the protein level decreased significantly (P = 0.01 )in TGF-β1 group,which was abolished in FOD group (P =0.03 )and 5-FU group (P = 0.02 ).The expression of vimentin at the protein level increased significantly (P =0.01)in TGF-β1 group,which was abolished in FOD group (P =0.04)and 5-FU group (P =0.03)and more obviously in FOD+5-FU group (P =0.01).Conclusion FOD can reverse the invasion of MHCC97-H cells in EMT induced by TGF-β1 through decreasing the expression of E-cadherin protein and inhibiting the epithelial-mesenchymal transition of MHCC97-H cell.关键词
MHCC97-H 细胞/TGF-β1/上皮间质转化/白花蛇舌草总黄酮/侵袭/E-cadherin/vimentinKey words
MHCC97-H cell/transforming growth factor-β1 (TGF-β1 )/epithelial-mesenchymal transition/total flavones of oldenlandia diffusa willd/invasion/E-cadherin/vimentin分类
医药卫生引用本文复制引用
张彦兵,朱娇,肖菊香,郭亚焕,廖子君,徐瑞..白花蛇舌草总黄酮对TGF-β1诱导的肝癌MHCC97-H细胞EMT的逆转作用及其机制[J].西安交通大学学报（医学版）,2016,(2):279-282,306,5.基金项目
陕西省社会发展攻关计划项目(No.2013K12-07-12) Supported by Social Development Research Plan of Shaanxi Province (No.2013K12-07-12) （No.2013K12-07-12）
