烟草科技Issue(1)：22-30,9.DOI:10.16135/j.issn1002-0861.20160104
纳米银对烟草花叶病毒（TMV）的抗性及作用机理
Preliminary experiments on nano-silver against tobacco mosaic virus and its mechanism
摘要
Abstract
In order to develop new efficient and low toxic biological pesticides against tobacco mosaic virus (TMV), chitosan nano-silver (cos-Ag) and chitosan Schiff-base nano-silver (S-cos-Ag) were synthesized. For investigating their inhibition effects against TMV, lesion experiments were conducted and resistance-related indexes were determined by using Xanthi-NN and Nicotiana tabacum cv. Qinyan96 as the test materials. The results showed that: 1) Thirty-fold diluted S-cos-Ag solution possessed better prevention and treatment efficacies against TMV and significantly reduced the amount of lesions in TMV-infected tobacco leaves, achieving a control efficacy of 78.33%. 2) Thirty-fold diluted S-cos-Ag solution promoted the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and phenylalanine ammonia lyase (PAL) in the tobacco leaves to different extents, leading to increased contents of chlorophyll and soluble protein while decreased malonaldehyde (MDA) contents. These results indicated that thirty-fold diluted S-cos-Ag could induce the TMV resistance on the tobacco and promote its immunity, thereby reducing the harm of TMV to tobacco.关键词
普通烟草/烟草花叶病毒(TMV)/席夫碱纳米银(S-cos-Ag)/壳寡糖纳米银(cos-Ag)/抗性Key words
Nicotiana tabacum/Tobacco mosaic virus (TMV)/Schiff-base nano-silver/Chitosan nano-silver/Resistance分类
农业科技引用本文复制引用
王莹,孙翠红,徐翠莲,王志敏,赵铭钦,王彩霞,刘玲玲,陈发元..纳米银对烟草花叶病毒（TMV）的抗性及作用机理[J].烟草科技,2016,(1):22-30,9.基金项目
河南省烟草公司资助项目“预防烟草病毒病的新型生物制剂应用技术与示范”(HYKJ201015)；“预防烟草花叶病毒病的生物制剂纳米增效研究”(HYKJ201307)。 （HYKJ201015）
