云南化工Issue(1)：6-10,5.DOI:10.3969/j.issn.1004-275X.2016.01.002
酶解－连续流动法在烟梗果胶含量测定中的应用
The Hydrolysis -continuous Flow Method Applied in Determination of Stem Pectin Content
摘要
Abstract
In order to provide a reference for determining pectin in tobacco stem exactly,the applica-bility for the determination of stem pectin content by Hydrolysis - continuous flow method was studied, meanwhile the hydrolysis -continuous flow method was developed,and the enzymatic hydrolysis conditions were optimized for 15 mg of pectinase at 55 ℃ for 3 h in 60mL of sodium citrate /citric acid (pH4.0)buffer solution by single factor experiments during the research.Under this condition,the method has good linearity with a correlation coefficient of 0.999 9,while the recovery of pectin in tobacco stem ranged from 97.8% to 100.3%,and the RSD was 1.06% to 2.43% intraday,1.87% during the day.It showed that the method was satisfactory and could be used for the determination of pectin in tobacco stem simultaneously.关键词
烟梗/果胶/酶解-连续流动法/定量测定Key words
tobacco stem/pectin/hydrolysis -continuous flow method/quantitative determination分类
轻工纺织引用本文复制引用
徐永明,梅键,周沅桢,潘纯祥,陶自伟,张朗,陈国辉,杨蕾..酶解－连续流动法在烟梗果胶含量测定中的应用[J].云南化工,2016,(1):6-10,5.基金项目
红云红河烟草（集团）有限责任公司资金支持项目，电子鼻检测烟叶霉变技术在制丝复烤线的应用研究（HYHH2013GY01）。 （）
