中国康复理论与实践Issue(1)：27-31,5.DOI:10.3969/j.issn.1006-9771.2016.01.006
莫诺苷对局灶性脑缺血再灌注大鼠皮层CD34表达的影响
Effects of Morroniside on Expression of CD34 in Ipsilateral Cortex of Focal Cerebral Ischemia-reperfusion Rats
摘要
Abstract
Objective To explore the effects of morroniside on the expression of CD34 in ipsilateral cortex of rats after focal cerebral isch-emia-reperfusion. Methods 45 male Sprague-Dawley rats were divided into sham group (n=9), ischemia group (n=9), and morroniside groups (low, medium and high dosage groups, n=9). The middle cerebral artery were occluded for 30 minutes, and reperfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg, 270 mg/kg after operation. The expression of CD34 in the isch-emic ipsilateral cortex were detected with immunohistochemistry (n=6) and Western blotting (n=3) 7 days after operation. Results The ex-pression of CD34 increased in the ischemia group compared with the sham group, and further increased in the morroniside groups of high dos-age compared with the ischemia group (F>14.865, P<0.001). Conclusion Morroniside could increase the expression of CD34 in the ischemic ipsilateral cortex after ischemia-reperfusion in rats, which may promote the angiogenesis and neurogenesis after ischemia.关键词
脑缺血再灌注/莫诺苷/CD34/血管新生Key words
cerebral ischemia-reperfusion/morroniside/CD34/angiogenesis分类
医药卫生引用本文复制引用
王志敏,郑文荣,王宇峰,郭德玉,王文,孙芳玲,刘婷婷,程华,向本旭,魏仁平,艾厚喜,田欣,祝自新..莫诺苷对局灶性脑缺血再灌注大鼠皮层CD34表达的影响[J].中国康复理论与实践,2016,(1):27-31,5.基金项目
1.“重大新药创制”科技重大专项(No.2012ZX09102201-106)；2.国家自然科学基金项目(No.81173575 （No.2012ZX09102201-106）
No.81373994)。 （）
